Strain information  NBRP Rat No: 0986  Strain name: F344-Cybbem1Shmo  Commmon Name: Rat Genome Database Principal Investigator:  Masayuki Mori  Shinshu University       3-1-1 Asahi, Matsumoto    390-8621      Japan Tel: 0263-37-2692    Fax: 0263-37-3428 Email: masamori@shinshu-u.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color   Inbred Generations   Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected NADPH oxidase 2 (Nox2) Origin A 7-base deletion was introduced into the first exon of the NADPH oxidase 2 (Nox2) gene of F344/DuCrj rats by CRISPR/Cas9. They were then backcrossed to F344/N (Japan SLC, Inc.). Strain characteristics Approximately 20% of female rats from 13 weeks of age onwards develop eosinophilia. It does not occur in male rats. Breeding Conditions Can be maintained by sibling mating between homozygous rats. Genotyping PCR, sequencing. Nox2-F：5′-GGAAACAGCCTTTCAGTTGG-3′ Nox2-R：5′-TCTCTAAGTAGATGTTTGTTCACAGAG-3′ References Cyba and Nox2 mutant rats show different incidences of eosinophilia in the genetic background- and sex-dependent manner. Mori M, Dai J, Miyahara H, Li Y, Kang X, Yoshimi K, Mashimo T, Higuchi K, Matsumoto K. Exp Anim. 2022 Nov 29. doi: 10.1538/expanim.22-0122. Online ahead of print. Additional strain information