Strain information
 NBRP Rat No: 0988  Strain name: F344-Cybbem1ShmoNox1em1ShmoNox4em1Shmo  Commmon Name: Rat Genome Database
Principal Investigator:  Masayuki Mori  Shinshu University       3-1-1 Asahi, Matsumoto    390-8621      Japan
Tel: 0263-37-2692    Fax: 0263-37-3428 Email: masamori@shinshu-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  アルビノ
Inbred Generations  
Usage Restrictions  
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected NADPH oxidase2 (Nox2), NADPH oxidase1 (Nox1), NADPH oxidase4 (Nox4)
Origin A 7-base deletion in the first exon of the NADPH oxidase 2 (Nox2) gene, a 29-base deletion in the first exon of the NADPH oxidase 1 (Nox1) gene, and a one-base insertion in the first exon of the NADPH oxidase 4 (Nox4) gene were introduced by CRISPR/Cas9 in the F344/DuCrj rats. They were then backcrossed to F344/N (Japan SLC, Inc.).
Strain characteristics Approximately 20% of female rats from 13 weeks of age onwards develop eosinophilia. It does not occur in male rats.
Breeding Conditions Can be maintained by sibling mating between homozygous rats.
Genotyping PCR, sequencing. Nox2-F：5′-GGAAACAGCCTTTCAGTTGG-3′ Nox2-R：5′-TCTCTAAGTAGATGTTTGTTCACAGAG-3′ Nox1-F：5′-TTCCGTCTACAGGAGGAAGG-3′ Nox1-R：5′-AGGCCAATAACTGCTACAGACA-3′ Nox4-F：5′-ATCTCCTGCCTGGCCTCTA-3′ Nox4-R：5′-CCCCAAACTTCCAATCCTCT-3′
References Cyba and Nox2 mutant rats show different incidences of eosinophilia in the genetic background- and sex-dependent manner.
Mori M, Dai J, Miyahara H, Li Y, Kang X, Yoshimi K, Mashimo T, Higuchi K, Matsumoto K.
Exp Anim. 2022 Nov 29. doi: 10.1538/expanim.22-0122. Online ahead of print.
Additional strain information