Strain information  NBRP Rat No: 1030  Strain name: F344-Il2rgem1Larc  Commmon Name: F344-Il2rg KO Δ1869 Rat Genome Database Principal Investigator:  Tomoji Mashimo  The University of Tokyo, The Institute of Medical Science       4-6-1 Shirokanedai-Minatoku-Tokyoto    108-8639      Japan Tel: 03-6409-2489    Fax:  Email: mashimo@ims.u-tokyo.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  アルビノ Inbred Generations  F2 Usage Restrictions  The following specific terms and conditions are requested by the DEPOSITOR. In this event, the recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. For commercial use, a separate agreement with the depositor must be concluded in addition to this consent form. Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others 免疫不全  Control Strain  Marker Strain Gene Affected Il2rg Origin This strain was generated at the Institute of Medical Science, University of Tokyo, by using CRISPR-Cas3. Microinjected into pronuclear-stage rat embryos of the F344/Jcl strain. The embryos were transferred into the oviducts of pseudopregnant females. A strain with a 1,869 bp deletion in the Il2rg gene was established from the resulting litters. Strain characteristics Breeding Conditions Mating heterozygous and wild-type rats. Genotyping Can be detected by PCR and electrophoresis. References Yoshimi K, Kuno A, Yamauchi Y, Hattori K, Taniguchi H, Mikamo K, Iida R, Ishida S, Goto M, Takeshita K, Ito R, Takahashi R, Takahashi S, Mashimo T. Genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes. Cell Rep Methods. 2024. Additional strain information