Strain information
 NBRP Rat No: 1008  Strain name: F344-ROSA26em11(CAG-EGFP,-mCherry)Larc  Commmon Name: F344-ROSA26-CAG-EGFP/mCherry Rat Genome Database
Principal Investigator:  Tomoji Mashimo  The University of Tokyo, The Institute of Medical Science       4-6-1 Shirokanedai-Minatoku-Tokyoto    108-8639      Japan
Tel: 03-6409-2489    Fax:  Email: mashimo@ims.u-tokyo.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  アルビノ
Inbred Generations  F6
Usage Restrictions  For commercial use, a contract must be concluded with the depositor in addition to this agreement.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others レポーター遺伝子動物
 Control Strain  Marker Strain
Gene Affected ROSA26
Origin This line was created at the Institute of Medical Science, University of Tokyo using the CRISPR-Cas9 method. F344/Jcl rat fertilized eggs were microinjected and transplanted into the oviducts of pseudopregnant females. From the resulting offspring, rats with the CAG promoter-loxP-EGFP-loxP-polyA-mCherry sequence inserted into the ROSA26 gene were selected and established.
Strain characteristics EGFP is expressed throughout the body, and when the EGFP sequence is removed in the presence of cre recombinase, mCherry is expressed.
Breeding Conditions Maintained by crossing heterozygous rats with wild type rats.
Genotyping The genotype is determined by PCR and electrophoresis.
References Ishida S, Taguchi K, Iida R, Hattori K, Taketsuru H, Yoshimi K, Yamamoto M, Mashimo T.
Diverse Cre recombinase expression pattern in Albumin-Cre driver rats.
Exp Anim. 2025 Jul 11;74(3):328-334.
Additional strain information