Strain information  NBRP Rat No: 1008  Strain name: F344-ROSA26em11(CAG-EGFP,-mCherry)Larc  Commmon Name: F344-ROSA26-CAG-EGFP/mCherry Rat Genome Database Principal Investigator:  Tomoji Mashimo  The University of Tokyo, The Institute of Medical Science       4-6-1 Shirokanedai-Minatoku-Tokyoto    108-8639      Japan Tel: 03-6409-2489    Fax:  Email: mashimo@ims.u-tokyo.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  アルビノ Inbred Generations  F6 Usage Restrictions  For commercial use, a contract must be concluded with the depositor in addition to this agreement. Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others レポーター遺伝子動物  Control Strain  Marker Strain Gene Affected ROSA26 Origin This line was created at the Institute of Medical Science, University of Tokyo using the CRISPR-Cas9 method. F344/Jcl rat fertilized eggs were microinjected and transplanted into the oviducts of pseudopregnant females. From the resulting offspring, rats with the CAG promoter-loxP-EGFP-loxP-polyA-mCherry sequence inserted into the ROSA26 gene were selected and established. Strain characteristics EGFP is expressed throughout the body, and when the EGFP sequence is removed in the presence of cre recombinase, mCherry is expressed. Breeding Conditions Maintained by crossing heterozygous rats with wild type rats. Genotyping The genotype is determined by PCR and electrophoresis. References Ishida S, Taguchi K, Iida R, Hattori K, Taketsuru H, Yoshimi K, Yamamoto M, Mashimo T. Diverse Cre recombinase expression pattern in Albumin-Cre driver rats. Exp Anim. 2025 Jan 22. Additional strain information