Strain information
 NBRP Rat No: 1017  Strain name: LE.W-Tg(Dbh-tTA,-cre)2_7Fusa/Koba  Commmon Name: DBH-Cre-BACTg2_7_917 Rat Genome Database
Principal Investigator:  Kazuto Kobayashi  Fukushima Medical University       1 Hikarigaoka Fukushima-shi    960-1295 Fukushima     Japan
Tel: 024-547-1667    Fax: 024-548-3936 Email: kazuto@fmu.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  白黒
Inbred Generations  F10 (July 2023)
Usage Restrictions  The user requires a collaboration with the depositor. Consult with the depositor about details beforehand.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 神経科学全般
 Control Strain  Marker Strain
Gene Affected DBH promoter, TetR, VP16 protein minimal“F”-type activation domain, 2A, Cre
Origin Background strain: Long-Evans (Institute for Animal Reproduction) Transgenes: DBH (rat), TetR (Escherichia coli), VP16 protein minimal "F"-type activation domain (Herpes simplex virus), 2A (Thosea asigna virus), Cre (P1 phage), bGlobin polyA (rabbit), loxP (P1 phage) The W-Tg(Dbh-tTA-2A-cre)2_7Fusa strain was provided by Dr. Fusao Kato and Dr. Yukari Takahashi of the Jikei University School of Medicine, and backcrossed to Long-Evans.
Strain characteristics Tetracycline-controlled transactivator (tTA), 2A peptide, and Cre recombinase are exogenously integrated downstream of the rat dopamine β-hydroxylase (DBH) promoter. By infecting these animals with a virus vector carrying the loxP sequence or mating with animals carrying the loxP sequence, recombination reaction by the Cre-loxP system can be induced specifically in DBH-positive noradrenergic and adrenergic neurons.
Breeding Conditions Good reproductive performance. Heterozygotes carrying the transgene are bred with Long Evans wild-type rats to maintain the line.
Genotyping The Cre sequence is recognized by PCR, and positive individuals are selected. Primer sequence Cre-F: GCAGAACCTGAAGATGTTCGCGAT, Cre-R: AGGTATCTCTGACCAGAGTCATCC Reaction solution: Milli Q up to 10 μl・10x Ex buffer 1 μl・dNTP 1 μl・Primer Cre-F (10 pmol/ul) 0.2 μl・Primer Cre-R (10 pmol/ul) 0.2 μl・DNA 0.5 μl・Ex Taq Hot start (TAKARA) 0.075 μl/Total 10 μl Program: 95°C, 3 min; (95°C, 30 sec; 60°C, 30 sec; 72°C, 1 min) x 35; 72°C, 7 min; 10°C, pause Product: Cre; 656 bp
References
Additional strain information