Strain information
 NBRP Rat No: 0961  Strain name: SD-P2rx4em18Taoki  Commmon Name: P2rx4-KO Rat Genome Database
Principal Investigator:  Tomohiro Aoki  The Jikei University School of Medicine       Building F, 3rd floor, 3-25-8, Nishi-shinbashi Minato-ku,    105-8461 Tokyo     Japan
Tel: 03-3433-1111    Fax:  Email: tomoaoki@jikei.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  白
Inbred Generations  不明
Usage Restrictions  In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature Designated by the DEPOSITOR is requested.
Isao Ono, Masahiko Itani, Akihiro Okada, Kimiko Yamamoto, Akitsugu Kawashima, Yoshiki Arakawa, Tomohiro Aoki
Pharmacological inhibition of P2RX4 receptor as a potential therapeutic strategy to prevent intracranial aneurysm formation
Exp Neurol. 2025 Feb:384:115061. doi: 10.1016/j.expneurol.2024.115061. Epub 2024 Nov 16.

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Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected P2rx4
Origin The purinergic receptor P2X4 (P2rx4)-deficient rat line was established at the National Cerebral and Cardiovascular Center Laboratory using the CRISPR/Cas9 system. SD rat (slc:SD) is a background strain. In line No. 18, conventional sequencing revealed genome deletion from the promoter region to the middle portion of exon 1 (171 b.p.) .
Strain characteristics No particular abnormalities are observed under normal rearing conditions. The purinergic receptor P2X4 binds ATP and functions as a channel that allows Ca2+ to flow into the cell, and also functions as a shear stress sensor in vascular endothelial cells.
Breeding Conditions Breeding is performed by mating between homozygous deficiencies is possible.
Genotyping Determined by PCR. KOD One PCR Master Mix (#KMM-101, Toyobo CO., LTD., Osaka, Japan) was used. Primer sets; forward 5'-CGAGCTGACCCTGCTCCCTGG-3', reverse 5'-CCCTGCGCAGTCCCTTCACC-3' Size; wild type; 732 b.p. mutant; 561 b.p.
References Isao Ono, Masahiko Itani, Akihiro Okada, Kimiko Yamamoto, Akitsugu Kawashima, Yoshiki Arakawa, Tomohiro Aoki
Pharmacological inhibition of P2RX4 receptor as a potential therapeutic strategy to prevent intracranial aneurysm formation
Exp Neurol. 2025 Feb:384:115061. doi: 10.1016/j.expneurol.2024.115061. Epub 2024 Nov 16.
Additional strain information