| NBRP Rat No: 0882 |
Strain name: STOCK Ugt2em1Yakaz Tc(UGT2-MAC)1Yakaz/Yakaz |
Commmon Name: U2M/U2KO, STOCK Tc (UGT2-MAC)-UGT2 |
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| Principal Investigator: |
Yasuhiro Kazuki Tottori University 86 Nishimachi 683-8503 Yonago-shi　Tottori Japan |
| Tel: 0859-38-6219 Fax: 0859-38-6211 |
Email: kazuki@tottori-u.ac.jp |
| Preservation Status: |
Embryo Sperm Living Animals |
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| Coat Color |
white |
| Inbred Generations |
F4 |
| Usage Restrictions |
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written approval from the DEPOSITOR using an Approval Form.
The RECIPIENT must inform the DEPOSITOR whether its research plan includes breeding, crossing with other strains and/or modifications of an inserted DNA.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for an academic research.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
| Genetic Status |
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| Comercial Availability |
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| Research Category |
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| Gene Affected |
UGT2 (human) or Ugt2 (Rat) cluster: cluster gene of UDP glucuronosyltransferase family 2 member |
| Origin |
Ugt2 (cluster) KO strain: The Ugt2 cluster was disrupted using the genome editing technology CRISPR/Cas9, and F1 rats (heterozygotes) were produced by crossing with Wistar (Crlj:WI) rats. The F1 rats were then crossed with each other to produce a homozygous strain.
Ugt2 KO map
Tc (UGT2-MAC) strain (Tc: transchromosomic): After introducing UGT2-MAC into rat ES cells (BLK2i-1), chimera rats were produced, which were then crossed with Wistar rats to produce F1 transmission rats, and further crossed with Wistar rats for several generations to produce strain rats. The MAC vector consists of the following genes: HPRT gene full length sequence, bovine growth hormone (BGH) polyA signal, b-actin promoter, b-globin insulator, CMV-IE enhancer, SV40 polyA signal, loxP sequence, FRT sequence, ampicillin resistance gene, neomycin resistance gene, kanamycin resistance gene, puromycin resistance gene, green fluorescent protein (GFP) gene and its mutants, PGK promoter, mouse chromosome 11 centromere region
Tc (UGT2-MAC)-UGT2 strain: The strain was created by crossing the Tc(UGT2-MAC) strain with the Ugt2 (cluster) KO strain.
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| Strain characteristics |
There is no significant phenotypic diferrence between normal rats and this strain. |
| Breeding Conditions |
Homozygous maintenance of Tc is not lethal, but it tends to be sterile and cannot be maintained by successive generations. Heterozygous maintenance of Tc. The transmission rate of Tc from females to offspring is about 50%, and from males to offspring is about 30%, so it is preferable to maintain it from females. |
| Genotyping |
Genotyping protocol for UGT2 |
| References |
Y Kazuki, K Kobayashi, M Hirabayashi, S Abe, N Kajitani, K Kazuki, S Takehara, M Takiguchi, D Satoh, J Kuze, T Sakuma, T Kaneko, T Mashimo, M Osamura, M Hashimoto, R Wakatsuki, R Hirashima, R Fujiwara, T Deguchi, A Kurihara, Y Tsukazaki, N Senda, T Yamamoto, N Scheer, M Oshimura
Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism
Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):3072-3081. |
| Additional strain information |
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