| NBRP Rat No: 1045 |
Strain name: WKY.Z-Leprfa/Ta |
Commmon Name: |
|
| Principal Investigator: |
Yoshikazu Ootani Takeda Pharmaceutical Company Limited 26-1, Muraoka-Higashi 2-chome, Fujisawa-shi 251-8555 Kanagawa Japan |
| Tel: 070-2687-9898 Fax: 0466-29-4462 |
Email: yoshikazu.ootani@takeda.com |
| Preservation Status: |
Embryo Sperm Living Animals |
 |
|
| Coat Color |
albino（c） |
| Inbred Generations |
N10F? |
| Usage Restrictions |
|
| Genetic Status |
|
| Comercial Availability |
|
|
| Research Category |
|
| Gene Affected |
Lepr: leptin receptor |
| Origin |
A congenic rat in which the leptin receptor gene Lepr on chromosome 5 derived from Zucker fatty rats was introduced by backcrossing for 10 generations into the genetic background of Wistar Kyoto rats (specific strain name unknown). By introducing the Lepr gene of Zucker fatty rats, which develop obesity but have normal blood glucose levels, into the genetic background of Wistar Kyoto rats, which have poorer glucose tolerance, the rats developed both obesity and diabetes. It was created by Ikeda et al. of Takeda Pharmaceutical Co., Ltd. and reported in 1981. |
| Strain characteristics |
obesity, diabetes, hyperglycemia, hyperinsulinemia, hypertriglyceridemia, insulin resistance |
| Breeding Conditions |
Since Lepr homozygotes are sterile in both sexes, mating is required between male and female heterozygotes. |
| Genotyping |
The Lepr genotype can be identified by PCR-RFLP using the restriction enzyme MspI. Primer-F: 5'-TATGGAAGTCACAGATGATGG-3', Primer-R: 5'-CTTACGATTGTAGAATTCTCTAA-3'. |
| References |
Ikeda H, Shino A, Matsuo T, Iwatsuka H, Suzuoki Z
A new genetically obese-hyperglycemic rat (Wistar fatty).
Diabetes. 1981 Dec;30(12):1045-50.
|
| Additional strain information |
|
|