Strain information
 NBRP Rat No: 0902  Strain name: LEW-Aqp4em1Mysi  Commmon Name: アクアポリン４ノックアウトラット
Principal Investigator:  Masato Yasui  Keio University       35 Shinnoucyo-Shinjyuku    160-8582 Tokyo     Japan
Tel: 03-5363-3750    Fax: 03-3359-8889 Email: yoabe@keio.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  albino
Inbred Generations  F2
Usage Restrictions  The following specific terms and conditions are requested by the DEPOSITOR. In this case, the receipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the article designated by the DEPOSITORS is required.
D Kato, H Kameda, N Kinota, T Fujii, B Xiawei, Z Simi, Y Takai, S Chau, Y Miyasaka, T Mashimo, Y Abe, M Yasui, K Minowa & K Kudo
Loss of aquaporin-4 impairs cerebrospinal fluid solute clearance through cerebrospinal fluid drainage pathways
Sci Rep. 2024 Nov 14;14(1):27982. doi: 10.1038/s41598-024-79147-y.
The RECIPIENT's use of the BIOLOGICAL RESOURCE shall be non-commercial research purpose only.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Aqp4
Origin This strain was generated during the process of producing AQP4 humanized rats (LEW-Aqp4em2Mysi ) by the group of Dr. Tomoji Mashimo, Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University in 2017. As a result of genome editing of LEW/CrlCrlj rats using the CRISPR/Cas9 system, a 295 bp deletion was made from the middle of exon 1 to the intron (from part of the Ser52 codon to Thr149 and 3 bases in the intron). The founder was backcrossed to LEW/CrlCrlj rats once, and then homozygous rats were obtained by crossing between heterozygotes.
Strain characteristics No significant phenotype is observed macroscopically.
Breeding Conditions Maintained by mating of homozygous rats.
Genotyping Both ends of the deletion site are amplified by PCR. sense: 5'-TCCTCAATGTCCCTTCCACTGGAGGCTTCTCATGC-3' antisense: 5'-ACCCAGGACCCCATGCGTGCAGGCAAGCGGTCTGC-3'
References D Kato, H Kameda, N Kinota, T Fujii, B Xiawei, Z Simi, Y Takai, S Chau, Y Miyasaka, T Mashimo, Y Abe, M Yasui, K Minowa & K Kudo
Loss of aquaporin-4 impairs cerebrospinal fluid solute clearance through cerebrospinal fluid drainage pathways
Sci Rep. 2024 Nov 14;14(1):27982.
Additional strain information