Strain information
 NBRP Rat No: 0905  Strain name: LEW-Aqp4em2Mysi  Commmon Name: アクアポリン４ヒト化ラット Rat Genome Database
Principal Investigator:  Masato Yasui  Keio University       35 Shinnoucyo-Shinjyuku    160-8582 Tokyo     Japan
Tel: 03-5363-3750    Fax: 03-3359-8889 Email: yoabe@keio.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  アルビノ
Inbred Generations  F2
Usage Restrictions  The following specific terms and conditions are requested by the DEPOSITOR. In this case, the receipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the article designated by the DEPOSITORS is required.
C Namatame, Y Abe, Y Miyasaka, Y Takai, Y Matsumoto, T Takahashi, T Mashimo, T Misu, K Fujihara, M Yasui, M Aoki
Humanized-Aquaporin-4-Expressing Rat Created by Gene-Editing Technology and Its Use to Clarify the Pathology of Neuromyelitis Optica Spectrum Disorder
Int J Mol Sci. 2024 Jul 26;25(15):8169. doi: 10.3390/ijms25158169.
The RECIPIENT's use of the BIOLOGICAL RESOURCE shall be non-commercial research purpose only.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Aqp4
Origin This strain was generated by the group of Dr. Tomoji Mashimo, Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University in 2017. Genome editing technology, the CRISPR/Cas9 system was used on LEW/CrlCrlj rats to replace five amino acids in exon 1 with human sequences (V53L, S62T, N64K, T120A, T149M) by homologous recombination using 1086 bp LssDNA. The founder was backcrossed to LEW/CrlCrlj rats once, and then homozygous rats were obtained by crossing between heterozygotes.
Strain characteristics No significant phenotype is observed macroscopically.
Breeding Conditions Maintained by mating of both homozygous rats.
Genotyping Based on the difference in sequence, the WT allele and humanized allele are amplified by PCR. WT sense: 5'-CATTAACTGGGGTGGCTCAGAGAACCCCCTACCTG-3' WT antisense: 5'-GCACAGAAATAAGAACAGAAGAGCTCTCACCGTG-3' humanize sense: 5'-CACCATTAACTGGGGTGGCACAGAGAAGCCTTTGC-3' humanize antisense: 5'-CAGCTCCGATGATGGCCCCGAGGCACTGCGCTGCG-3'
References C Namatame, Y Abe, Y Miyasaka, Y Takai, Y Matsumoto, T Takahashi, T Mashimo, T Misu, K Fujihara, M Yasui, M Aoki
Humanized-Aquaporin-4-Expressing Rat Created by Gene-Editing Technology and Its Use to Clarify the Pathology of Neuromyelitis Optica Spectrum Disorder
Int J Mol Sci. 2024 Jul 26;25(15):8169.
Additional strain information