Strain information
 NBRP Rat No: 1036  Strain name: LIS-Tyrem1(EGFP)Mabe  Commmon Name: Rat Genome Database
Principal Investigator:  Manabu Abe  Niigata University       1-757 Asahimachidori, Chuo-Ku, Niigata    951-8585 Niigata     Japan
Tel: 025-227-0621    Fax: 025-227-0816 Email: manabu@bri.niigata-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  アルビノ
Inbred Generations  
Usage Restrictions  In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Abe M, Nakatsukasa E, Natsume R, Hamada S, Sakimura K, Watabe AM, Ohtsuka T.
A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes.
Sci Rep. 2023 Feb 8;13(1):2245.

In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgement to the DEPOSITOR is requested.
The following specific terms and conditions are requested by the DEPOSITOR. In this event, the recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
If a patent application is field as a result of research using resource, prior consent must be obtained from the depositor (or a notice to that effect must be field).
For commercial use, a separate agreement with the depositor must be concluded in addition to this consent form.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Tyr
Origin This strain was generated at Brain Research Institute, Niigata University using the CRISPR-Cas9 method. It was established by electroporating fertilized eggs of Lister Hooded rats (Seac:LIS) using the rGONAD method and selecting rats in which the EGFP sequence was inserted into the Tyr gene sequence.
Strain characteristics Because the EGFP sequence was inserted into the Tyr gene sequence, the coat color is albino and the rat expresses EGFP.
Breeding Conditions Maintained by crossing heterozygotes with wild type rats.
Genotyping Can be identified by PCR, electrophoresis, and sequencing as described in the reference. Abe M, Nakatsukasa E, Natsume R, Hamada S, Sakimura K, Watabe AM, Ohtsuka T. A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes. Sci Rep. 2023 Feb 8;13(1):2245.
References Abe M, Nakatsukasa E, Natsume R, Hamada S, Sakimura K, Watabe AM, Ohtsuka T.
A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes.
Sci Rep. 2023 Feb 8;13(1):2245.
Additional strain information