Strain information  NBRP Rat No: 0937  Strain name: WIC-Myod1em2Kykn  Commmon Name: MyoD-deficient (+1 bp) Rat Genome Database Principal Investigator:  Keitaro Yamanouchi  The University of Tokyo        1-1-1, Yayoi, Bunkyo-ku,    113-8657 Tokyo     Japan Tel: 03-5841-5386    Fax: 03-5841-8017 Email: akeita@g.ecc.u-tokyo.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  白色 Inbred Generations   Usage Restrictions  In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others 筋疾患（筋発生・筋再生）  Control Strain  Marker Strain Gene Affected MyoD Origin A mutation was introduced into the MyoD gene of Wistar-Imamichi rats (Iar: Wistar-Imamichi, Institute for Animal Reproduction) using the CRISPR/Cas9 method. A single base was inserted into Exon1. Strain characteristics MyoD-deficient rats (-/-) die neonatally within 24 hours after birth. Breeding Conditions Maintained by natural mating between heterozygous rats. Genotyping The genotype of each rat is examined by PCR. Primer F: ATTGTACTATTGGGGTTCAGGAGTG Primer R: CAGACCTTCAATGTAGCGGATG PCR conditions: KOD FX Neo is used. 94℃ 5 min x 1 cycle 98℃ 10 sec, 58℃ 30 sec, 68℃ 40 sec x 38 cycles. After PCR, the product is treated with the restriction enzyme Bsl1. A microchip electrophoresis device for DNA/RNA analysis (Multina) is used for analysis. Wild type: 154 bp, homozygous deletion: 123 bp, heterozygous: 154 bp and 123 bp digestion products are detected. References Additional strain information