Strain information
 NBRP Rat No: 1043  Strain name: LE-Oprm1em1(cre)Bfdi  Commmon Name:
Principal Investigator:  Yilong Cui  RIKEN Center for Biosystems Dynamics Research       Kobe 650-0047, Hyogo, Japan    650-0047      Japan
Tel: 078-304-7192    Fax: 078-304-7191 Email: cuiyl@riken.jp
Preservation Status:   Embryo        Sperm       Living Animals ../images/Photos/ ../images/Photos/
Coat Color  
Inbred Generations  F67
Usage Restrictions  In publishing, a citation of the following literatures designated by the DEPOSITOR is requested.
<Name of literatures>
Opioidergic activation of the descending pain inhibitory system underlies placebo analgesia
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected oprm1
Origin The genetic background is Iar: Long-Evans (Institute for Animal Reproduction). Long-Evans rats were introduced from Monash University to Institute for Animal Experimentation, Tohoku University in 1984, and then to Institute for Animal Reproduction in 1996. These rats were generated with the support of the Advanced Animal Model Support.
Strain characteristics Cre gene expression is dependent on the Oprm1 gene promoter.
Breeding Conditions Good breeding performance
Genotyping Genotyping by PCR. Protocol 50mM NaOH 180μl 100℃ 20 min → 1M Tris-HCL(PH8) 20μl → vortex → Spin down → Move supernatant to new tube DNA 0.3μl KAPA2 5μl Primer Forward (10μM) 0.2μl(final 0.2μM) Primer Reverse (10μM) 0.2μl (final 0.2μM) dH2O 4.3μl 10μl 1. 94℃ 1 min 2. 98℃ 10 sec 3.60℃ 15 sec 4. 68℃ 30 sec step2-4 30cycles 5. 72℃ 10 min 6. 4℃ hold 2% Agarose gel 100V 25 minutes Primer: F1: 5’-TGATGGCCCTCATTCTGAGC-3’ (R1) F2: 5’-GGCGCTAAGGATGACTCTGG-3’ (R2, 3) F3: 5’-TAGTTGCCAGCCATCTGTTG-3’ (F1) R1: 5’-TAACTACCTGTTTTGCCG GG-3’ (F2) R2: 5’-GACAGCAAGGGGGAGGAT-3’ (F2, 3) R3: 5’-ATGTGGGGGTTAGGAGAGGG -3’
References H Neyama, Y Wu, Y Nakaya, S Kato, T Shimizu, T Tahara, M Shigeta, M Inoue, K Miyamichi, N Matsushita, T Mashimo, Y Miyasaka, Y Dai, K Noguchi, Y Watanabe, M Kobayashi, K Kobayashi, Y Cui
Opioidergic activation of the descending pain inhibitory system underlies placebo analgesia
Sci Adv. 2025 Jan 17;11(3):eadp8494.
Additional strain information