Strain information  NBRP Rat No: 0093  Strain name: DOP/Nem  Commmon Name: DOP, dilute-opisthotonus Rat Genome Database Principal Investigator:  Yoshiko Takagishi  Research Institute of Environmental Medicine, Nagoya University       Furo-cho, Chigusa-ku, Nagoya    464－8601 Aichi     JAPAN Tel: 052-789-3874    Fax: 052-789-3876 Email: taka@riem.nagoya-u.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  gray Inbred Generations  F25+?+16 (May 2009) Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested (*). Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected Myo5a: myosin Va Origin Founded from a breeding colony of Wistar by Ohno at Yagi Memorial Park in 1988. A congenic line BN-dop and an inbred line DOP-dop were established. Strain characteristics Normal behaviour until day 10, thereafter, excessive spinning around on smooth surfaces and tendency to be off balance. Slight convulsive limb movements. Can't regain upright posture. Limb convulsion associated with opisthotonus becomes tetanic. Hyperacuety. Dead after 3 weeks. Lighter coat color pigmentation. Controlled by an autosomal recessive gene (Dekker-Ohno et al. 1993). Breeding Conditions Maintained by crossing heterozygotes of the dop locus (segregating inbred strain). Genotyping Genotyping protocol for Myo5 References * Dekker-Ohno K, Oda S, Yamamura H, Kondo K. An ataxic mutant rat with dilute coat color. Lab Anim Sci. 1993 Aug;43(4):370-2 * Futaki S, Takagishi Y, Hayashi Y, Ohmori S, Kanou Y, Inouye M, Oda S, Seo H, Iwaikawa Y, Murata Y. Identification of a novel myosin-Va mutation in an ataxic mutant rat, dilute-opisthotonus. Mamm Genome. 2000 Aug;11(8):649-55. * Dekker-Ohno K, Hayasaka S, Takagishi Y, Oda S, Wakasugi N, Mikoshiba K, Inouye M, Yamamura H. Endoplasmic reticulum is missing in dendritic spines of Purkinje cells of the ataxic mutant rat. Brain Res. 1996 Apr 1;714(1-2):226-30 * Miyata M, Finch EA, Khiroug L, Hashimoto K, Hayasaka S, Oda SI, Inouye M, Takagishi Y, Augustine GJ, Kano M. Local calcium release in dendritic spines required for long-term synaptic depression. Neuron. 2000 Oct;28(1):233-44 Additional strain information DOP