Strain information  NBRP Rat No: 0084  Strain name: F344.CVD-Unc5ccvd/Kyo  Commmon Name: F344.CVD-Unc5h3cvd, cerebellar vermis defect Rat Genome Database Principal Investigator:  Tadao Serikawa  Graduate School of Medicine, Kyoto University       Yoshidakonoe-cho, Sakyo-ku    606-8501 Kyoto     JAPAN Tel: 075-753-4360    Fax: 075-753-4409 Email: serikawa@anim.med.kyoto-u.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  albino (c) Inbred Generations  N12F16 (May 2009) Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected Unc5c: unc-5 homolog C (C. elegans) Origin Spontanious mutation from LEW inbred at Osaka Prefecture University in 1992. A congenic strain was produced by backcrosses to F344/NSlc strain at Kyoto University. Strain characteristics Hereditary cerebellar vermis defect. Mutant rats exhibit hind-leg paralysis from about 14 days old. Gross pathology showes the cerebellar vermis defect, fused cerebellar hemispheres and cyst formation. Ectopic dysplastic cerebellar tissues exist in the cerebello-pontine junctional zones. Mild disarrangement of lamination was also observed in the fused cerebellar hemispheres (Kuwamura 1994). Breeding Conditions Good breeding performance. Sib mating between homorozygous female and heterozygous male. Genotyping Genotyping protocol for Unc5c References Kuramoto T, Kuwamura M, Serikawa T. Rat neurological mutations cerebellar vermis defect and hobble are caused by mutations in the netrin-1 receptor gene Unc5h3. Brain Res Mol Brain Res. 122(2): 103-108, 2004. Kuwamura M, Yoshida T, Yamate J, Kotani T, Sakuma S. Hereditary cerebellar vermis defect in the Lewis rat. Dev Brain Res. 83: 294-298, 1994. Kuwamura M, Yoshida T, Ishida A, Tsudzuki M, Yamate J, Kotani T, Sakuma S. Cerebellar vermis defect: a new neurological mutation in the rat. J Hered. 87(2): 161-162, 1996. Kuwamura M, Yoshida T, Yamate J, Kotani T, Sakuma S, Tsudzuki M. Perivascular external granule cells in hereditary cerebellar vermis defect rat: pathogenesis of cerebellar cortical dysplasia. J Vet Med Sci. 58(9): 875-879, 1996. Kuwamura M, Ishida A, Yamate J, Kato K, Kotani T, Sakuma S. Chronological and immunohistochemical observations of cerebellar dysplasia and vermis defect in the hereditary cerebellar vermis defect (CVD) rat. Acta Neuropathol. 94: 549-556, 1997. Kuwamura M, Shirota A, Yamate J, Kotani T, Sakuma S. Analysis of aberrant neuronal migrations in the hereditary cerebellar vermis defect (CVD) rat using bromodeoxyuridine-immunohistochemistry. Acta Neuropathol. 95: 143-148, 1998. Kuwamura M, Morikawa T, Yamate J, Kato K, Kotani T, Sakuma S. Glial pathology in development of cerebellar dysplasia in the hereditarycerebellar vermis defect (CVD) rat. Acta Neuropathol. 99: 305-309, 2000. Kuwamura M, Shirota A, Takada A, Yamate J, Kotani T, Sakuma S. Spontaneous and ethyl-nitrosourea-induced medullomyoblastomas in cerebellarvermis defect (CVD) mutant rat. Acta Neuropathol. 99: 285-288, 2000. Additional strain information F344 CVD