Strain information
NBRP Rat No: 1048
Strain name
: LE-
Pvalb
em2(flpo)Koba
Commmon Name: PV-Flpo-2
Principal Investigator:
Kazuto Kobayashi Fukushima Medical University 1 Hikarigaoka Fukushima-shi 960-1295 Fukushima Japan
Tel: 024-547-1667 Fax: 024-548-3936
Email: kazuto@fmu.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
白黒
Inbred Generations
F7 (March 2025)
Usage Restrictions
The user requires a collaboration with the depositor. Consult with the depositor about details beforehand.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Pvalb (parvalbumin)
Origin
Background strain: Long-Evans (Iar:Long-Evans, Institute for Animal Reproduction). Using the CRISPR/Cas9 system (Combi-CRISPR), the "T2A-Flpo" sequence was inserted into the stop codon within the fourth exon of the parvalbumin (Pvalb) gene in fertilized rat eggs. In the PVA-Flpo-2 strain, a two-base deletion and a single-base substitution (underline) were observed in the third intron of the Pvalb gene due to NHEJ after a double-strand break induced by Combi-CRISPR. Target site sequence: Wild type: TTGGCGGGCCAGAACCTCAG(GGG) Knock-in strain: TTGGC
A
GGCCAGAACーーCAG(GGG) ( ) indicates the PAM sequence.
Strain characteristics
Flpo is expressed in accordance with the spatiotemporal expression of Pvalb.
Breeding Conditions
Good breeding performance. Maintained by mating heterozygous rats with Long-Evans rats.
Genotyping
Forward primer: 5'-GTGCATTTCCAGAGGTGACAG (within the third intron) Reverse primer: 5'-GGTGGTGTCCGATGGGTACAG (within the 3'UTR) Amplified fragment size: Wild-type: 421 bp; Knock-in: 1778 bp In heterozygotes, both wild-type and knock-in amplifications were detected simultaneously.
References
準備中
Additional strain information