Strain information
NBRP Rat No: 1050
Strain name
: LE-Tg(Tac1-IL2RA/YFP*)2-6Koba
Commmon Name: Tac1-IL-2Ra-mVenus RecBAC2-6
Principal Investigator:
Kazuto Kobayashi Fukushima Medical University 1 Hikarigaoka Fukushima-shi 960-1295 Fukushima Japan
Tel: 024-547-1667 Fax: 024-548-3936
Email: kazuto@fmu.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
白黒
Inbred Generations
F16 (March 2025)
Usage Restrictions
The user requires a collaboration with the depositor. Consult with the depositor about details beforehand.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
IL2R: alpha-subunit, Tac1: preprotachykinin-A
Origin
Background strain: Long-Evans (Iar:Long-Evans, Institute for Animal Reproduction). This rat strain was generated by injecting a modified BAC, in which a fusion protein made from the IL2R alpha-subunit gene and YFP was inserted into the second exon of the rat Tac1 gene, into fertilized eggs of Long-Evans rats.
Strain characteristics
A transgenic rat expressing a fusion protein constructed from the IL2RA alpha-subunit gene and YFP under the control of Tac1.
Breeding Conditions
Good breeding performance. Maintained by mating heterozygous rats with Long-Evans rats.
Genotyping
"Positive individuals were selected by recognizing the IL2RA sequence by PCR. Primer sequence: F: ATGGATTCATACCTGCTGAT R: GCTACCTGGTACTCTGTTGT PCR conditions: Enzyme: TaKaRa Taq 94℃ 5 min → 94℃(30 cycles) 20 sec → 56℃ 30 sec → 72℃ 30 sec → 4℃ infinity Amplified fragment size: 724 bp"
References
準備中
Additional strain information