Strain information
NBRP Rat No: 1051
Strain name
: LE-
Tac1
em2(IL2RA)Koba
Commmon Name: Tac1-IL2R knock-in rats
Principal Investigator:
Kazuto Kobayashi Fukushima Medical University 1 Hikarigaoka Fukushima-shi 960-1295 Fukushima Japan
Tel: 024-547-1667 Fax: 024-548-3936
Email: kazuto@fmu.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
白黒
Inbred Generations
F4 (March 2025)
Usage Restrictions
The user requires a collaboration with the depositor. Consult with the depositor about details beforehand.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
Tac1 (tachykinin, precursor 1) , IL2RA/ヒトHA-tag/ヒトインフルエンザウイルスT2A/Thosea asignaウイルス
Origin
Background strain: Long-Evans (Iar:Long-Evans, Institute for Animal Reproduction). Details of the introduced gene: This rat strain was generated by inserting the "IL2RA/HA-T2A" sequence into the start codon (methionine) within the second exon of the Tachykinin precursor 1 (Tac1) gene in fertilized rat eggs using the CRISPR/Cas9 system (Combi-CRISPR). A single-base deletion is observed in the first intron of the Tac1 gene due to NHEJ after a double-strand break induced by Combi-CRISPR. Target site sequence: Wild-type: GTTTGGAGAAATGTTCGACC(TGG) Knock-in strain: GTTTGGAGAAATGTTCーACC(TGG) ( ) indicates the PAM sequence.
Strain characteristics
IL2RA/HA is expressed under the control of the Tac1 promoter.
Breeding Conditions
Good breeding performance. Maintained by mating heterozygous rats with Long-Evans rats.
Genotyping
Forward primer: 5'-CGTCAGTAGCCTTCTTAAAGGGT (within the first intron) Reverse primer: 5'-GTTCTGGGCAATAAGCCTTGTG (within the second intron) Amplified fragment size: Wild-type: 463 bp; Knock-in: 1399 bp In heterozygotes, both wild-type and knock-in amplifications are detected simultaneously.
References
準備中
Additional strain information