Strain information
 NBRP Rat No: 1053  Strain name: SD-Brca2em18Tykn  Commmon Name:
Principal Investigator:  Shinya Toyokuni  Nagoya University Graduate School of Medicine       65 Tsurumai-cho, Showa-ku, Nagoya    466-8550 Aichi     Japan
Tel: 052-741-2087    Fax: 052-744-2091 Email: yashiro.m@hotmail.co.jp
Preservation Status:   Embryo        Sperm       Living Animals ../images/Photos/ ../images/Photos/
Coat Color  Albino
Inbred Generations  F9以降
Usage Restrictions  In publishing, a citation of the following literature designated by the DEPOSITOR is requested.　
Heterozygous mutation in BRCA2 induces accelerated age-dependent decline in sperm quality with male subfertility in rats.
Sci Rep. 2025 Jan 2;15(1):447. doi: 10.1038/s4 1598-024-84 184-8.

The following specific terms and conditions are requested by the DEPOSITOR.
1.The Recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
2.The Recipient shall use the BIOLOGICAL RESOURCE in collaborative research with the DEPOSITOR. The DEPOSITOR shall be included as a co-author in any resulting publication.
3.If the Recipient intends to file a patent or other intellectual property based on the results obtained through the use of the BIOLOGICAL RESOURCE, the Recipient shall contact the DEPOSITOR in advance and shall discuss the handling of such application.
4.For any use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT shall reach an agreement with the DEPOSITOR on the terms and conditions of such use and shall obtain the DEPOSITOR’s prior written consent.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Brca2
Origin This strain was created in collaboration with Professor Tomoji Mashimo of the University of Tokyo. Genome editing was performed using CRISPR/Cas9 on fertilized eggs of SD rats, targeting exon 11 of the Brca2 gene, to introduce a frameshift with a stop codon. The genetic background is Jcl:SD.
Strain characteristics Brca2 exon 11 contains a frameshift/stop codon in the seventh BRC repeat. Specifically, Brca2 p.T1942fs is homologous to human BRCA2 p.T1974fs. Homozygotes for this mutation are embryonic lethal. Sperm DNA fragmentation is observed in heterozygotes even at a young age. Furthermore, a decline in reproductive efficiency is seen in both males and females around eight months of age.
Breeding Conditions Maintained by mating heterozygous males with wild-type Jcl:SD females purchased from a provider.
Genotyping Can be detected by PCR and electrophoresis.
References Motooka Y, Tanaka H, Maeda Y, Katabuchi M, Mashimo T, Toyokuni S.
Heterozygous mutation in BRCA2 induces accelerated age-dependent decline in sperm quality with male subfertility in rats.
Sci Rep. 2025 Jan 2;15(1):447.

Tanaka H, Motooka Y, Maeda Y, Sonehara R, Nakamura T, Kajiyama H, Mashimo T, Toyokuni S.
Brca2(p.T1942fs/+) dissipates ovarian reserve in rats through oxidative stress in follicular granulosa cells.
Free Radic Res. 2024 Feb;58(2):130-143.
Additional strain information