Strain information  NBRP Rat No: 1063  Strain name: F344-Mocosem1Tua  Commmon Name: Mocos Line20 Principal Investigator:  Takashi Kuramoto  Tokyo University of Agriculture       1737 Funako, Atsugi,    243-0034 Kanagawa     Japan Tel: 046-270-6583    Fax: 046-270-6585 Email: tk206782@nodai.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  albino Inbred Generations  F7 (2025.6.19) Usage Restrictions  The following specific terms and conditions are requested by the DEPOSITOR. In this event, the recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. For commercial use, a separate agreement with the depositor must be concluded in addition to this consent form. Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected Mocos Origin This rat strain was generated using the CRISPR/Cas9 system in an F344/Jcl embryo and contains a nonsense mutation (AGG>TGA, R419X) in exon 7 of the Mocos gene. Strain characteristics Molybdenum cofactor sulfurase (MOCOS) is an enzyme that adds sulfur to molybdenum coenzymes. The sulfurized molybdenum coenzyme conjugates with xanthine oxidase (XDH) and metabolizes purines. Specifically, it oxidizes hypoxanthine and xanthine, ultimately producing uric acid. This rat carries the R419X nonsense mutation in the Mocos gene. Homozygous individuals exhibit low body weight, polydipsia and polyuria, xanthinuria, and renal failure, and all die by 16 weeks of age. Breeding Conditions Homozygotes cannot be used for breeding. They are maintained by mating between heterozygotes. Genotyping "Determine the induced mutation site (AGG>TGA) using Cycleave PCR method. Sequences of primer to obtain amplicon are 5′-CACAACCAGACGTTTCTGAC-3′ and 5′-ACTACAGGGTCTTGCACTGA-3′, respectively. Probe to detect the wild-type allele is 5′-CACCTG(A)GG-3′ in which 3′ is labelled with FAM. Probe to detect the mutant allele is 5′- CAGTTC(A)CAG-3′ in which 3′ is labelled with ROX. RNA in the probe is enclosed with parentheses. Real-time PCR is performed with CycleavePCR Reaction Mix (Takara Bio, Inc., Kusatsu, Shiga, Japan)." References Urasaki M, Nagasaka K, Kido M, Hayashi K, Watanabe A, Hattori K, Sekiguchi T, Kuwamura M, Tanaka M, Mashimo T, Kuramoto T. A nonsense mutation in the Mocos gene induces xanthinuria, obstructive nephropathy, and anemia in rats Exp Anim. 2025 Dec 3. Additional strain information