Strain information  NBRP Rat No: 0140  Strain name: F344/Stm  Commmon Name: Rat Genome Database Principal Investigator:  Ryoji Hokao  Institute for Animal Reproduction       1103, Fukaya, Kasumigaura,     300-0131 Ibaraki     Japan Tel: 029-897-0631    Fax: 029-897-0633 Email: hokao_ryoji@iar.or.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  albino (a,B,c,h,R) Inbred Generations  F64+27 (April 2012) Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected Origin F344/Stm rats were derived from a pair of F344/DuCrj rats purchased from Charles River Japan, Inc. and thereafter maintained by brother-sister matings for >23 generations in Saitama Cancer Center Research Institute. Strain characteristics A parental strain of LEXF/FXLE RI strains (NBRP No.0105-0138). This strain has a loss-of-function misssense mutation (Gly633Arg) in DIPEPTIDYL PEPTIDASE IV（Dpp4）gene as F344/DuCrlCrlj strain. (July 7, 2016) Breeding Conditions Good breeding performance. Sib mating. Genotyping References Numakura Y, Uemura R, Tanaka M, Izawa T, Yamate J, Kuramoto T, Kaneko T, Mashimo T, Yamamoto T, Serikawa T, Kuwamura M. PHF24 is expressed in the inhibitory interneurons in rats. Exp Anim. 2021 Feb 6;70(1):137-143. Hoang Trung H, Yoshihara T, Nakao A, Hayashida K, Hirata Y, Shirasuna K, Kuwamura M, Nakagawa Y, Kaneko T, Mori Y, Asano M, Kuramoto T. Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats. Sci Rep. 2021 Jan 21;11(1):2039. Ueda Y, Hirota K, Yamauchi I, Hakata T, Yamashita T, Fujii T, Yasoda A, Inagaki N. Is C-type natriuretic peptide regulated by a feedback loop? A study on systemic and local autoregulatory effect. PLoS One. 2020 Oct 1;15(10):e0240023. Kim H, Yoshihara M, Suyama M. Comparative genomic analysis of inbred rat strains reveals the existence of ancestral polymorphisms. Mamm Genome. 2020 Apr;31(3-4):86-94. Serikawa T, Kunisawa N, Shimizu S, Kato M, Alves Iha H, Kinboshi M, Nishikawa H, Shirakawa Y, Voigt B, Nakanishi S, Kuramoto T, Kaneko T, Yamamoto T, Mashimo T, Sasa M, Ohno Y. Increased seizure sensitivity, emotional defects and cognitive impairment in PHD finger protein 24 (Phf24)-null rats. Behav Brain Res. 2019 Sep 2:369:111922. Fujii T, Hirota K, Yasoda A, Takizawa A, Morozumi N, Nakamura R, Yotsumoto T, Kondo E, Yamashita Y, Sakane Y, Kanai Y, Ueda Y, Yamauchi I, Yamanaka S, Nakao K, Kuwahara K, Jindo T, Furuya M, Mashimo T, Inagaki N, Serikawa T, Nakao K. Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death. PLoS One. 2018 Mar 22;13(3):e0194812. Meek S, Mashimo T, Burdon T. From engineering to editing the rat genome. Mamm Genome. 2017 Aug;28(7-8):302-314. Yoshihara M, Sato T, Saito D, Ohara O, Kuramoto T, Suyama M. Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains. Genomics Data. 2016 Dec; 10: 155-157 Taketsuru H, Kaneko T. In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability. J Reprod Dev. 2016 Jul 2. [Epub ahead of print] Nakanishi S, Serikawa T, Kuramoto T. Slc:Wistar Outbred Rats Show Close Genetic Similarity with F344 Inbred Rats. Exp Anim. 64(1):25-29, 2015. Kaneko T, Mashimo T. Creating Knockout and Knockin Rodents Using Engineered Endonucleases via Direct Embryo Injection. Methods Mol Biol. 1239:307-315, 2015. Yoshimi K, Kaneko T, Voigt B, Mashimo T. Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat Commun. 5:4240, 2014. Kaneko T, Sakuma T, Yamamoto T, Mashimo T. Simple knockout by electroporation of engineered endonucleases into intact rat embryos. Sci Rep. 2014 Oct 1:4:6382. Taketsuru H, Kaneko T. Efficient collection and cryopreservation of embryos in F344 strain inbred rats. Cryobiology. 2013 Oct;67(2):230-4. Voigt B, Kuramoto T, Mashimo T, Tsurumi T, Sasaki Y, Hokao R, Serikawa T. Evaluation of LEXF/FXLE rat recombinant inbred strains for genetic dissection of complex traits. Physiol Genomics. 2008 Feb 19;32(3):335-42. Hashimoto R, Voigt B, Ishimaru Y, Hokao R, Chiba S, Serikawa T, Sasa M, Kuramoto T. Identification of QTLs involved in the development of amygdala kindling in the rat. Exp Anim. 2013;62(3):181-7. Shisa, H. et al. The LEXF:a new set of rat recombinant inbred strains between LE/Stm and F344. Mamm, Genome.8:324-327(1997). Lu, L-M., Shisa H. et al. Propynitrosourea-induced T-lymphomas in LEXF RI strains of rats:genetic analysis. Br. J.Cancer. 80:855-861(1999) Additional strain information F344