Strain information
 NBRP Rat No: 0197  Strain name: KDP-Tg(H2Kd-Cblb)2Nyo  Commmon Name: Rat Genome Database
Principal Investigator:  Susumu Seino  Kobe University Graduate School of Medicine       7-5-1 Kusunoki-cho, Chuou-Ku, Kobe    650-0017 Hyogo     Japan
Tel: 078-382-5364    Fax: 078-382-5379 Email: seino@med.kobe-u.ac.jp, yokoi@med.kobe-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  black hooded (a,B,C,h)
Inbred Generations  N5
Usage Restrictions  The recipients shall cite the references of the resource and acknowledge the developer as the source of the resource in the publications. Depending on the project of the recipients, a collaborative arrangement with the developer could be required. In this case, the recipients shall discuss with the developer. The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Cblb: Casitas B-lineage lymphoma b
Origin The transgenic rats were established by injection of transgene consisting of wild-type Cblb into Sprague Dawley (Slc:SD) rat fertile eggs in 2000, followed by backcrossing the transgenic rats onto KDP rats. This transgenic rat expresses the wild-type cDNA of Cblb gene under the control of mouse H2-Kd promoter. (Jul 2009)
Strain characteristics KDP is a spontaneous animal model of human type 1 diabetes and a nonsense mutation in Cblb was identified as a causative gene. To confirm that the Cblb mutation is responsible for the phenotype of the KDP rat, wild-type Cblb gene was introduced to KDP rats as rescue experiments. As a result, this transgenic strain was rescued partially (about 30% diabetes incidence as show in KDP) (Yokoi, 2002). (Jul 2009)
Breeding Conditions Homozygous Cblb rats are usually infertile in both sexes and are therefore maintained by crossing hemizygous individuals (segregating inbred strain).
Genotyping
References Yokoi N, Komeda K, Wang HY, Yano H, Kitada K, Saitoh Y, Seino Y, Yasuda K, Serikawa T, Seino S.
Cblb is a major susceptibility gene for rat type 1 diabetes mellitus.
Nat Genet. 31(4):391-4, 2002

Yokoi N, Namae M, Fuse M, Wang HY, Hirata T, Seino S, Komeda K.
Establishment and characterization of the Komeda diabetes-prone rat as a segregating inbred strain.
Exp Anim. 52(4):295-301, 2003
Additional strain information KDP