Strain information  NBRP Rat No: 0273  Strain name: W-Tg(CAG-GFP)184Ys  Commmon Name: Wistar-Tg(CAG-GFP)184Ys, Wistar-TgN(CAG-GFP)184Ys, Wistar GFP Rat Genome Database Principal Investigator:  Eiji Kobayashi  Center for Molecular Medicine, Jichi Medical School       3311-1, Yakushiji, Shimono-shi, Tochigi    329-0498 Tochigi     Japan Tel: 0285-58-7446    Fax: 0285-44-5365 Email: eijikoba@jichi.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  albino (c) Inbred Generations  F21 (March 2012) Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected GFP: Green Fluorescent Protein, jellyfish Origin This strain was established at YS institute and maintained at PhoenixBio Co., Ltd. and Jichi Medical School. This transgenic rat was originated from Crlj:WI strain. (May 25, 2010) Strain characteristics This transgenic strain expresses the enhanced green fluorescent protein (EGFP) ubiquitously driven by CAG promoter. Breeding Conditions Good breeding performance. Genotyping Hakamata Y et al., Biochem Biophys Res Commun 2001, 286:779-85 Sense 5'-CCTACGGCAAGCTGACCCTGAAGTT-3' Anti-sense 5'-AGGACCATGTGATCGCGCTTCTCGT-3'" References Matsuda Y, Sasaki M, Kataoka-Sasaki Y, Takayanagi A, Kobayashi K, Oka S, Nakazaki M, Masumori N, Kocsis JD, Honmou O. Intravenous Infusion of Bone Marrow-Derived Mesenchymal Stem Cells Reduces Erectile Dysfunction Following Cavernous Nerve Injury in Rats. Sex Med. 2017 Dec 20. pii: S2050-1161(17)30097-1. [Epub ahead of print] Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Oka S, Namioka T, Namioka A, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Mikami T, Wanibuchi M, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells inhibits intracranial hemorrhage after recombinant tissue plasminogen activator therapy for transient middle cerebral artery occlusion in rats. J Neurosurg. 2017 Jan 6:1-10. Sasaki Y, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Suzuki J, Tateyama D, Oka S, Namioka T, Namioka A, Onodera R, Mikami T, Wanibuchi M, Kakizawa M, Ishiai S, Kocsis JD, Honmou O. Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats. Phys Ther. 2016 Nov;96(11):1791-1798. Morita T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Oka S, Oshigiri T, Takebayashi T, Yamashita T, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury. Neuroscience. 2016 Oct 29;335:221-31. Anna Labedz-Maslowska, Elzbieta Kamycka, Sylwia Bobis-Wozowicz, Zbigniew Madeja, and Ewa K. Zuba-Surma. Identification of new rat bone marrow- derived population of very small stem cell with Oct-4A and Nanog expression by flow cytometric platforms. Stem Cells International. 2015. Article ID 531098 Moriyama Y, Takagi N, Itokawa C, Tanonaka K. Injection of neural progenitor cells attenuates decrease in level of connexin 43 in brain capillaries after cerebral ischemia. Neurosci Lett. 2013 May 24;543:152-6. Hernández Vera R, Vilahur G, Ferrer-Lorente R, Peña E, Badimon L. Platelets derived from the bone marrow of diabetic animals show dysregulated endoplasmic reticulum stress proteins that contribute to increased thrombosis. Arterioscler Thromb Vasc Biol. 2012 Sep;32(9):2141-8. Y Moriyama, N Takagi and K Tanonaka Intravenous injection of neural progenitor cells improved depression-like behavior after cerebral ischemia Translational Psychiatry (2011) 1, e29 Green fluorescent protein-transgenic rat: a tool for organ transplantation research. Hakamata Y, Tahara K, Uchida H, Sakuma Y, Nakamura M, Kume A, Murakami T, Takahashi M, Takahashi R, Hirabayashi M, Ueda M, Miyoshi I, Kasai N, Kobayashi E. Biochem Biophys Res Commun. 2001 Aug 31;286(4):779-85. Intravenous injection of neural progenitor cells improved depression-like behavior after cerebral ischemia Y Moriyama, N Takagi and K Tanonaka Translational Psychiatry (2011) 1, e29 Offspring derived from intracytoplasmic injection of transgenic rat sperm. Hirabayash M, Kato M, Aoto T, Sekimoto A, Ueda M, Miyoshi I, Kasai N, Hochi S. Transgenic Res. 2002 Apr;11(2):221-8. Lympho-myeloid chimerism achieved by spleen graft of green fluorescent protein transgenic rat in a combined pancreas transplantation model (TI03-029). Sakuma Y, Sato Y, Inoue S, Kaneko T, Hakamata Y, Takahashi M, Murakami T, Kobayashi E. Transpl Immunol. 2004 Jan;12(2):115-22. Morphologic characterization of green fluorescent protein in embryonic, neonatal, and adult transgenic rats. Takeuchi K, Sereemaspun A, Inagaki T, Hakamata Y, Kaneko T, Murakami T, Takahashi M, Kobayashi E, Ookawara S. Anat Rec A Discov Mol Cell Evol Biol. 2003 Oct;274(2):883-6. Long-lasting donor passenger leukocytes after hepatic and intestinal transplantation in rats. Inoue S, Tahara K, Kaneko T, Ajiki T, Takeda S, Sato Y, Hakamata Y, Murakami T, Takahashi M, Kaneko M, Kobayashi E. Transpl Immunol. 2004 Jan;12(2):123-31. Additional strain information Wistar