Strain information  NBRP Rat No: 0273  Strain name: W-Tg(CAG-GFP)184Ys  Commmon Name: Wistar-Tg(CAG-GFP)184Ys, Wistar-TgN(CAG-GFP)184Ys, Wistar GFP Rat Genome Database Principal Investigator:  Eiji Kobayashi  Center for Molecular Medicine, Jichi Medical School       3311-1, Yakushiji, Shimono-shi, Tochigi    329-0498 Tochigi     Japan Tel: 0285-58-7446    Fax: 0285-44-5365 Email: eijikoba@jichi.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  albino (c) Inbred Generations  F21 (March 2012) Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected GFP: Green Fluorescent Protein, jellyfish Origin This strain was established at YS institute and maintained at PhoenixBio Co., Ltd. and Jichi Medical School. This transgenic rat was originated from Crlj:WI strain. (May 25, 2010) Strain characteristics This transgenic strain expresses the enhanced green fluorescent protein (EGFP) ubiquitously driven by CAG promoter. Breeding Conditions Good breeding performance. Genotyping Hakamata Y et al., Biochem Biophys Res Commun 2001, 286:779-85 Sense 5'-CCTACGGCAAGCTGACCCTGAAGTT-3' Anti-sense 5'-AGGACCATGTGATCGCGCTTCTCGT-3'" References Terada K, Sasaki M, Nagahama H, Kataoka-Sasaki Y, Oka S, Ukai R, Yokoyama T, Iizuka Y, Sakai T, Fukumura S, Tsugawa T, Kocsis JD, Honmou O. Therapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury. Pediatr Res. 2023 Dec;94(6):1921-1928. Maeda S, Kawamura T, Sasaki M, Shimamura K, Shibuya T, Harada A, Honmou O, Sawa Y, Miyagawa S. Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model. Sci Rep. 2022 Oct 10;12(1):16986. Tabata H, Sasaki M, Kataoka-Sasaki Y, Shinkai N, Ichihara K, Masumori N, Kocsis JD, Honmou O. Possible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat. BMC Urol. 2021 Nov 13;21(1):156. Matta A, Karim MZ, Gerami H, Benigno B, Erwin WM. A comparative study of mesenchymal stem cell transplantation and NTG-101 molecular therapy to treat degenerative disc disease. Sci Rep. 2021 Jul 20;11(1):14804. Magota H, Sasaki M, Kataoka-Sasaki Y, Oka S, Ukai R, Kiyose R, Onodera R, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells delays disease progression in the SOD1G93A transgenic amyotrophic lateral sclerosis rat model. Brain Res. 2021 Apr 15:1757:147296. Yasuda N, Sasaki M, Kataoka-Sasaki Y, Nagahama H, Kocsis JD, Kawaharada N, Honmou O. Intravenous delivery of mesenchymal stem cells protects both white and gray matter in spinal cord ischemia. Brain Res. 2020 Nov 15:1747:147040. Goto T, Miyagawa S, Tamai K, Matsuura R, Kido T, Kuratani T, Shimamura K, Sakaniwa R, Harada A, Sawa Y. High-mobility group box 1 fragment suppresses adverse post-infarction remodeling by recruiting PDGFRα-positive bone marrow cells. PLoS One. 2020 Apr 10;15(4):e0230392. Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Oka S, Suzuki J, Sasaki Y, Nagahama H, Hashi K, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells improves impaired cognitive function in a cerebral small vessel disease model. Neuroscience. 2019 Jun 1:408:361-377. Sakai T, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Fukumura S, Kobayashi M, Tsutsumi H, Kocsis JD, Honmou O. Functional recovery after the systemic administration of mesenchymal stem cells in a rat model of neonatal hypoxia-ischemia. J Neurosurg Pediatr. 2018 Nov 1;22(5):513-522. Namioka A, Namioka T, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells for protection against brainstem infarction in a persistent basilar artery occlusion model in the adult rat. J Neurosurg. 2018 Oct 12;131(4):1308-1316. Matsuda Y, Sasaki M, Kataoka-Sasaki Y, Takayanagi A, Kobayashi K, Oka S, Nakazaki M, Masumori N, Kocsis JD, Honmou O. Intravenous Infusion of Bone Marrow-Derived Mesenchymal Stem Cells Reduces Erectile Dysfunction Following Cavernous Nerve Injury in Rats. Sex Med. 2017 Dec 20. pii: S2050-1161(17)30097-1. [Epub ahead of print] Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Oka S, Namioka T, Namioka A, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Mikami T, Wanibuchi M, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells inhibits intracranial hemorrhage after recombinant tissue plasminogen activator therapy for transient middle cerebral artery occlusion in rats. J Neurosurg. 2017 Jan 6:1-10. Sasaki Y, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Suzuki J, Tateyama D, Oka S, Namioka T, Namioka A, Onodera R, Mikami T, Wanibuchi M, Kakizawa M, Ishiai S, Kocsis JD, Honmou O. Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats. Phys Ther. 2016 Nov;96(11):1791-1798. Morita T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Oka S, Oshigiri T, Takebayashi T, Yamashita T, Kocsis JD, Honmou O. Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury. Neuroscience. 2016 Oct 29;335:221-31. Anna Labedz-Maslowska, Elzbieta Kamycka, Sylwia Bobis-Wozowicz, Zbigniew Madeja, and Ewa K. Zuba-Surma. Identification of new rat bone marrow- derived population of very small stem cell with Oct-4A and Nanog expression by flow cytometric platforms. Stem Cells International. 2015. Article ID 531098 Moriyama Y, Takagi N, Itokawa C, Tanonaka K. Injection of neural progenitor cells attenuates decrease in level of connexin 43 in brain capillaries after cerebral ischemia. Neurosci Lett. 2013 May 24;543:152-6. Hernández Vera R, Vilahur G, Ferrer-Lorente R, Peña E, Badimon L. Platelets derived from the bone marrow of diabetic animals show dysregulated endoplasmic reticulum stress proteins that contribute to increased thrombosis. Arterioscler Thromb Vasc Biol. 2012 Sep;32(9):2141-8. Y Moriyama, N Takagi and K Tanonaka Intravenous injection of neural progenitor cells improved depression-like behavior after cerebral ischemia Translational Psychiatry (2011) 1, e29 Green fluorescent protein-transgenic rat: a tool for organ transplantation research. Hakamata Y, Tahara K, Uchida H, Sakuma Y, Nakamura M, Kume A, Murakami T, Takahashi M, Takahashi R, Hirabayashi M, Ueda M, Miyoshi I, Kasai N, Kobayashi E. Biochem Biophys Res Commun. 2001 Aug 31;286(4):779-85. Intravenous injection of neural progenitor cells improved depression-like behavior after cerebral ischemia Y Moriyama, N Takagi and K Tanonaka Translational Psychiatry (2011) 1, e29 Offspring derived from intracytoplasmic injection of transgenic rat sperm. Hirabayash M, Kato M, Aoto T, Sekimoto A, Ueda M, Miyoshi I, Kasai N, Hochi S. Transgenic Res. 2002 Apr;11(2):221-8. Lympho-myeloid chimerism achieved by spleen graft of green fluorescent protein transgenic rat in a combined pancreas transplantation model (TI03-029). Sakuma Y, Sato Y, Inoue S, Kaneko T, Hakamata Y, Takahashi M, Murakami T, Kobayashi E. Transpl Immunol. 2004 Jan;12(2):115-22. Morphologic characterization of green fluorescent protein in embryonic, neonatal, and adult transgenic rats. Takeuchi K, Sereemaspun A, Inagaki T, Hakamata Y, Kaneko T, Murakami T, Takahashi M, Kobayashi E, Ookawara S. Anat Rec A Discov Mol Cell Evol Biol. 2003 Oct;274(2):883-6. Long-lasting donor passenger leukocytes after hepatic and intestinal transplantation in rats. Inoue S, Tahara K, Kaneko T, Ajiki T, Takeda S, Sato Y, Hakamata Y, Murakami T, Takahashi M, Kaneko M, Kobayashi E. Transpl Immunol. 2004 Jan;12(2):123-31. Additional strain information Wistar