Strain information  NBRP Rat No: 0083  Strain name: F344.Cg-Foxn1rnu/Kyo  Commmon Name: F344/N-Foxn1rnu/Kyo, F344/N-rnu Rat Genome Database Principal Investigator:  Tadao Serikawa  Graduate School of Medicine, Kyoto University       Yoshidakonoe-cho, Sakyo-ku    606-8501 Kyoto     JAPAN Tel: 075-753-4360    Fax: 075-753-4409 Email: serikawa@anim.med.kyoto-u.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  albino (c,rnu) Inbred Generations  F?+57 (March 2012) Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected Foxn1: forkhead box N1 Origin This congenic strain was established as a strain with the genetic background of F344/N onto which a segment from the nude rat containing the Foxn1rnu was transferred. Originally, hairless mutant (rnu) was observed in a colony of outbred hooded rats maintained at the Rowett Research Institute in Scotland (Festing, 1978). Backcrossing started at Central Institute for Experimental Animals in 1979 and thereafter the subline was transported to the Institute of Laboratory Animals Graduate School of Medicine, Kyoto University. (Mar 25, 2010) Strain characteristics rnu/rnu lacks normal thymus and functionally mature T cells, and is characterized by hairlessness (thinning hair) but usually exhibits short and thin hairs and has hair cycle (Festing, 1978; Hedrich, 1990). Life span is shorter under the conventional condition compared to under the SPF condition. rnu/+ is smaller than rnu/rnu. rnu was mapped on rat chromosome 10 in the q24-q32 interval (Kuramoto, 1993), and identified as a nonsense mutation (1429C>T) of Foxn1 (alias Whn) gene (Serge, 1995). Similar mutant rat independently arose in a colony of randomly bred albino rats in New Xealand (Berridge, 1979、Nehls, 1994). (Mar 25, 2010) Breeding Conditions Good breeding performance. Sib mating between heterozygous female and homozygous male are mated. Genotyping Genotyping protocol for Foxn1 References Festing MF, May D, Connors TA, Lovell D, Sparrow S. An athymic nude mutation in the rat. Nature. 1978 Jul 27;274(5669):365-6. Vos JG, Kreeftenberg JG, Kruijt BC, Kruizinga W, Steerenberg P. The athymic nude rat. II. Immunological characteristics. Clin Immunol Immunopathol. 1980 Feb;15(2):229-37. Rolstad B. The athymic nude rat: an animal experimental model to reveal novel aspects of innate immune responses? Immunol Rev. 2001 Dec;184:136-44. Hedrich H Mutant genes and polymorphic loci of the laboratory rat. In H.J.Hedrich (ed.): Genetic Monitoring of the Inbred Strains of Rats, pp377-380, Gustav Fisher Stuttgart, New York 1990. Kuramoto T, Serikawa T, Hayasaka N, Mori M, Yamada J. Regional mapping of the Rowett nude gene (RONU) to rat chromosome 10q24-->q32 by localizing linked SYB2 and GH loci. Cytogenet Cell Genet. 1993;63(2):107-10. Segre JA, Nemhauser JL, Taylor BA, Nadeau JH, Lander ES. Positional cloning of the nude locus: genetic, physical, and transcription maps of the region and mutations in the mouse and rat. Genomics. 1995 Aug 10;28(3):549-59. Berridge MV, O'Kech N, McNeilage LJ, Heslop BF, Moore R. Rat mutant (NZNU) showing "nude" characteristics. Transplantation. 1979 Jun;27(6):410-3. Nehls M, Pfeifer D, Schorpp M, Hedrich H, Boehm T. New member of the winged-helix protein family disrupted in mouse and rat nude mutations. Nature. 1994 Nov 3;372(6501):103-7. Additional strain information F344