Strain information
 NBRP Rat No: 0352  Strain name: SD-Tg(Nes/Hspa1b-EGFP)Okn  Commmon Name: SD-Tg(Nes-Hspa1b-EGFP)Okn, Nestin-EGFP Rat Genome Database
Principal Investigator:  Hideyuki Okano  Keio University       35 Shinano-cho, Shinjyuku-ku    160-8582 Tokyo     Japan
Tel: 03-5363-3747    Fax: 03-3357-5445 Email: hidokano@sc.itc.keio.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  albino (c)
Inbred Generations  N3 (April 2012)
Usage Restrictions  
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Nes (nestin), Hspa1b (heat shock 70kD protein 1B), GFP (Green Fluorescent Protein, jellyfish)
Origin This transgenic strain was established at Slc in 1999 and transferred to Keio University in 2000.
Strain characteristics This strain expresses green fluorescent protein (GFP) neural-specific driven by Nestin enhancer and Hspa1b promoter.
Breeding Conditions Maintained in hemizygous condition. Usually, maintained by crossing of male transgenic rats and female wild-type rats.
Genotyping Transgenic rats were identified by Southern blot analysis or by PCR using tail DNA and primers specific for the GFP sequence.
References Sawamoto K, Nakao N, Kakishita K, Ogawa Y, Toyama Y, Yamamoto A, Yamaguchi M, Mori K, Goldman SA, Itakura T, Okano H.
Generation of dopaminergic neurons in the adult brain from mesencephalic precursor cells labeled with a nestin-GFP transgene.
J Neurosci. 2001 Jun 1;21(11):3895-903.
Additional strain information