Strain information  NBRP Rat No: 0352  Strain name: SD-Tg(Nes/Hspa1b-EGFP)Okn  Commmon Name: SD-Tg(Nes-Hspa1b-EGFP)Okn, Nestin-EGFP Rat Genome Database Principal Investigator:  Hideyuki Okano  Keio University       35 Shinano-cho, Shinjyuku-ku    160-8582 Tokyo     Japan Tel: 03-5363-3747    Fax: 03-3357-5445 Email: hidokano@sc.itc.keio.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  albino (c) Inbred Generations  N3 (April 2012) Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected Nes (nestin）, Hspa1b (heat shock 70kD protein 1B), GFP (Green Fluorescent Protein, jellyfish) Origin This transgenic strain was established at Slc in 1999 and transferred to Keio University in 2000. Strain characteristics This strain expresses green fluorescent protein (GFP) neural-specific driven by Nestin enhancer and Hspa1b promoter. Breeding Conditions Maintained in hemizygous condition. Usually, maintained by crossing of male transgenic rats and female wild-type rats. Genotyping Transgenic rats were identified by Southern blot analysis or by PCR using tail DNA and primers specific for the GFP sequence. References Sawamoto K, Nakao N, Kakishita K, Ogawa Y, Toyama Y, Yamamoto A, Yamaguchi M, Mori K, Goldman SA, Itakura T, Okano H. Generation of dopaminergic neurons in the adult brain from mesencephalic precursor cells labeled with a nestin-GFP transgene. J Neurosci. 2001 Jun 1;21(11):3895-903. Additional strain information