Strain information  NBRP Rat No: 0297  Strain name: LEW-Tg(CAG-EGFP)1Ys  Commmon Name: LEW-TgN(CAG-EGFP)1Ys, GFP LEW Rat Genome Database Principal Investigator:  Eiji Kobayashi  Center for Molecular Medicine, Jichi Medical School       3311-1, Yakushiji, Shimono-shi, Tochigi    329-0498 Tochigi     Japan Tel: 0285-58-7446    Fax: 0285-44-5365 Email: eijikoba@jichi.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  albino (c) Inbred Generations  F18 (March 2012) Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected GFP: Green Fluorescent Protein, jellyfish Origin This transgenic strain was established at YS institute. Strain characteristics This transgenic strain contains the enhanced green fluorescent protein (EGFP) gene ubiquitously driven by CAG promoter. Breeding Conditions Good breeding performance. Genotyping Genotyping protocol for CAG Promoter Genotyping protocol for EGFP References Esmaeili R, Sadeghpour A, Darbandi-Azar A, Majidzadeh-A K, Vajhi A, Sadeghizadeh M Echocardiographic assessment of myocardial infarction: comparison of a rat model in two strains. Iran J Vet Res. 2017 Winter;18(1):30-35. Kaizawa Y, Kakinoki R, Ikeguchi R, Ohta S, Noguchi T, Takeuchi H, Oda H, Yurie H, Matsuda S. A Nerve Conduit Containing a Vascular Bundle and Implanted With Bone Marrow Stromal Cells and Decellularized Allogenic Nerve Matrix. Cell Transplant. 2017 Feb 16;26(2):215-228. Maeda H, Okamoto K, Namikawa T, Tsuda M, Uemura S, Shiga M, Hanazaki K, Kobayashi M. Re-evaluation of hepatocyte replacement by recipient-derived cells after allogenic liver transplantation: Discrepancy between clinical observations and a rat model. Hepatol Res. 2016 Jan 4. [Epub ahead of print] Tomita K, Nishibayashi A, Yano K, Hosokawa K. Adipose-derived stem cells protect against endoneurial cell death: Cell therapy for nerve autografts. Microsurgery. 2015 Jul 20 [Epub ahead of print] Ohshima M, Taguchi A, Tsuda H, Sato Y, Yamahara K, Harada-Shiba M, Miyazato M, Ikeda T, Iida H, Tsuji M. Intraperitoneal and intravenous deliveries are not comparable in terms of drug efficacy and cell distribution in neonatal mice with hypoxia-ischemia. Brain Dev. 2015 Apr;37(4):376-386, 2015. Tomita Koichi, Nishibayashi Akimitsu, Yano Kenji, Hosokawa Ko. Differentiated Adipose-derived Stem Cells Promote Reinnervation of Rat Skin Flaps. Plast Reconstr Surg Glob Open 2013;1:e22 Ishikane S, Yamahara K, Sada M, Harada K, Kodama M, Ishibashi-Ueda H, Hayakawa K, Mishima K, Iwasaki K, Fujiwara M, Kangawa K, Ikeda T. Allogeneic administration of fetal membrane-derived mesenchymal stem cells attenuates acute myocarditis in rats. J Mol Cell Cardiol. 2010 Nov;49(5):753-61. Tsuda H, Yamahara K, Ishikane S, Otani K, Nakamura A, Sawai K, Ichimaru N, Sada M, Taguchi A, Hosoda H, Tsuji M, Kawachi H, Horio M, Isaka Y, Kangawa K, Takahara S, Ikeda T. Allogenic fetal membrane-derived mesenchymal stem cells contribute to renal repair in experimental glomerulonephritis. Am J Physiol Renal Physiol. 2010 Nov;299(5):F1004-13. Inoue H, Ohsawa I, Murakami T, Kimura A, Hakamata Y, Sato Y, Kaneko T, Takahashi M, Okada T, Ozawa K, Francis J, Leone P, Kobayashi E. Development of new inbred transgenic strains of rats with LacZ or GFP. Biochem Biophys Res Commun. 329(1):288-295, 2005. Additional strain information LEW