Gene Affected
Lyst: lysosomal trafficking regulator
Origin
Spontaneous mutation from ACI/NKyo inbred at Kyoto University in 1999.
Strain characteristics
This mutant rat shows beige coat color, giant granule cells in various tissues, and prolonged bleeding time. This mutant strain is a disease model for human Chediak-Higashi syndrome (CHS). This mutation is a deletion of about 20 kb containing exons 28, 29 and 30, which constitute the 578 bp sequence missing in the Lyst cDNA on rat chromosome 17. This mutant gene is allelic to Lystbe (DA-beige rat) but the putative deletion break points in L1 elements were different from it (Mori, 2003). The nested PCR-RFLP analysis is useful to discriminate Lystbe-Kyo from Lystbe (Masui, 2004). (May 21, 2009)
Breeding Conditions
Maintained by crossing of homozygoutes. Litter size is small.
References
山崎賢一、中西聡、北田一博、芹川忠夫、西川哲、並木千晶、浜田修一、橋本敦
ACI/N系統に出現した新たな淡毛色ラットの解析
第68回関西動物実験研究会要旨
Mori M, Yamasaki K, Nakanishi S, Kitada K, Higuchi K, Namiki C, Hamada S, Serikawa T.
A new beige mutant rat ACI/NKyo-Lyst
Exp. Anim. 52(1), 31-36, 2003.
Masui N, Mori M, Nishikawa T, Takagi Y, Asai H, Yanabe M, Yamasaki K, Serikawa T, Sato K.
An allele-specific genotyping method for rat lyst (lysosomal trafficking regulator) gene.
Exp Anim. 2004 53(1):77-80.