Strain information  NBRP Rat No: 0002  Strain name: ACI-Lystbg-Kyo/Kyo  Commmon Name: ACI/NKyo-Lystbg-Kyo/Kyo, ACI/NKyo-Lystbg-Kyo, ACI/N-bg Rat Genome Database Principal Investigator:  Tadao Serikawa  Graduate School of Medicine, Kyoto University       Yoshidakonoe-cho, Sakyo-ku    606-8501 Kyoto     JAPAN Tel: 075-753-4360    Fax: 075-753-4409 Email: serikawa@anim.med.kyoto-u.ac.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  beige (A,B,hi,bg) Inbred Generations  F35+F27 (May 2009) Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected Lyst: lysosomal trafficking regulator Origin Spontaneous mutation from ACI/NKyo inbred at Kyoto University in 1999. Strain characteristics This mutant rat shows beige coat color, giant granule cells in various tissues, and prolonged bleeding time. This mutant strain is a disease model for human Chediak-Higashi syndrome (CHS). This mutation is a deletion of about 20 kb containing exons 28, 29 and 30, which constitute the 578 bp sequence missing in the Lyst cDNA on rat chromosome 17. This mutant gene is allelic to Lystbe (DA-beige rat) but the putative deletion break points in L1 elements were different from it (Mori, 2003). The nested PCR-RFLP analysis is useful to discriminate Lystbe-Kyo from Lystbe (Masui, 2004). (May 21, 2009) Breeding Conditions Maintained by crossing of homozygoutes. Litter size is small. Genotyping References 山崎賢一、中西聡、北田一博、芹川忠夫、西川哲、並木千晶、浜田修一、橋本敦 ACI/N系統に出現した新たな淡毛色ラットの解析 第68回関西動物実験研究会要旨 Mori M, Yamasaki K, Nakanishi S, Kitada K, Higuchi K, Namiki C, Hamada S, Serikawa T. A new beige mutant rat ACI/NKyo-Lyst Exp. Anim. 52(1), 31-36, 2003. Masui N, Mori M, Nishikawa T, Takagi Y, Asai H, Yanabe M, Yamasaki K, Serikawa T, Sato K. An allele-specific genotyping method for rat lyst (lysosomal trafficking regulator) gene. Exp Anim. 2004 53(1):77-80. Additional strain information ACI