Strain information
 NBRP Rat No: 0469  Strain name: W-Tg(Gnrh1-EGFP)Nphy  Commmon Name: Wistar-Tg(Gnrh1-EGFP)Nphy, Wistar-Tg(GnRH-EGFP)Nphy Rat Genome Database
Principal Investigator:  Masakatsu Kato  Nippon Medical school       1-1-5, Sendagi, Bunkyo-ku    113-8602 Tokyo     Japan
Tel: 03-3822-2131　ext 5242    Fax: 03-5685-3055 Email: mkato@nms.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals ../images/Photos/WistarTg_GnRH_EGFP/Wistar-Tg_Gnrh-EGFP_a_1024.jpg ../images/Photos/WistarTg_GnRH_EGFP/GnRH_1024.jpg
Coat Color  albino (c)
Inbred Generations  F?+13 (April 2012)
Usage Restrictions   In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested. (see References)
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 神経生理学、神経内分泌学
 Control Strain  Marker Strain
Gene Affected GFP: Green Fluorescent Protein, jellyfish
Origin This trans genic strain was established in 2001.
Strain characteristics This transgenic strain contains the enhanced green fluorescent protein (EGFP) gene driven by gonadotropin-releasing hormone 1 (Gnrh1) promoter. EGFP fluorescence is observed only in Gnrh1-immunoreactive neurons, approximately one third of which has strong EGFP fluorescence.
Breeding Conditions Since high inbred generations reduce breeding performance, crossing to wild type is necessary once in a while.
Genotyping Genotyping protocol for Gnrh1-EGFP
References Kato M, Ui-Tei K, Watanabe M, Sakuma Y.
Characterization of voltage-gated calcium currents in gonadotropin-releasing hormone neurons tagged with green fluorescent protein in rats.
Endocrinology. 144(11):5118-25, 2003.

Yin C., Ishii H., Tanaka N., Sakuma Y., Kato M.
Activation of A-type g-amino butyric acid receptors (GABAARs) excites gonadotropin-releasing hormone (GnRH) neurones isolated from adult rats.
J Neuroendocrinol. 2008 May;20(5):566-75.

Kato M, Tanaka N, Usui S, Sakuma Y.
The SK channel blocker apamin inhibits slow afterhyperpolarization currents in rat gonadotropin-releasing hormone neurones.
J Physiol. 15;574(Pt 2):431-42, 2006.

Watanabe M., Sakuma Y., Kato M.
GABAA receptors mediated excitation in adult rat GnRH neurons.
Biol Reprod. 2009 Aug;81(2):327-332.

Kato M., Tanaka N., Ishii H., Yin C., Sakuma Y.
Ca2+ channels and Ca2+-activated K+ channels in adult rat gonadotrophin-releasing hormone neurones.
J Neuroendocrinol. 2009 Mar;21(4):312-315.

Tanaka N., Ishii H., Yin C., Koyama M., Sakuma Y., Kato M.
Voltage-gated Ca2+ channel mRNAs and T-type Ca2+ currents in rat gonadotropin-releasing hormone neurons.
J. Physiol. Sci. 2010 May;60(3):195-204.

Koyama M, Yin C, Ishii H, Sakuma Y, Kato M.
Somatostatin inhibition of GnRH neuronal activity and the morphological relationship between GnRH and somatostatin neurons in rats.
Endocrinology. 2012 Feb;153(2):806-814.

Tada H, Kuroki Y, Funabashi T, Kamiya Y, Goto T, Suyama K, Sano A, Mitsushima D, Etgen AM, Takahashi T.
Phasic synaptic incorporation of GluR2-lacking AMPA receptors at gonadotropin-releasing hormone neurons is involved in the generation of the luteinizing hormone surge in female rats.
Neuroscience. 2013 Sep 17;248:664-66
Additional strain information