Strain information
 NBRP Rat No: 0491  Strain name: WIC-Tg(Wap-GH1)2Mni  Commmon Name: WistarImamichi-Tg(Wap-GH1)2Mni, WistarImamichi-Tg(mWAP-hGH)2Mni, WAP/hGH-transgenic rat (low line) Rat Genome Database
Principal Investigator:  Masugi　Nishihara  Agricultural and Life Sciences ,The University of Tokyo       1-1-1 Bunkyo-ku, Yayoi    113-8657 Tokyo     Japan
Tel: 03-5841-5386    Fax: 03-5841-8017 Email: amnishi@mail.ecc.u-tokyo.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  albino (c)
Inbred Generations  
Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested. (see References)
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected GH1: growth hormone 1
Origin Ikeda developed transgenic rats carrying the human growth hormone (Gh1) driven by murine Wap promoter, originated from Wistar-Imamichi (Ikeda, 1994). After that this transgenic strain has been maintained by artificial insemination to wild-type Wistar-Imamichi. Two lines of this transgenic strain were established named Line 1: characterized by relatively high level of serum Gh1 (high line, NBRPNo.0490), and Line 2: relatively low level of serum Gh1 (low line, NBRPNo.0491). (Oct 30, 2009)
Strain characteristics This strain shows gigantism, polyposia, polyuria and insulin resistance in adipocytes and muscle. (Oct 30, 2009)
Breeding Conditions Since this strain’s natural reproductivity is very low, usually it is maintained by artificial insemination, taking sperms from male transgenic rats. (Oct 30, 2009)
Genotyping
References Ikeda A, Matsuyama S, Nishihara M, Tojo H, Takahashi M.
Changes in endogenous growth hormone secretion and onset of puberty in transgenic rats expressing human growth hormone gene.
Endocr J. 41(5):523-9, 1994.

Ikeda A, Matsumoto Y, Chang KT, Nakano T, Matsuyama S, Yamanouchi K, Ohta A, Nishihara M, Tojo H, Sasaki F, Takahashi M.
Different female reproductive phenotypes determined by human growth hormone (hGH) levels in hGH-transgenic rats.
Biol Reprod. 56(4):847-51, 1997.

Ikeda A, Chang KT, Matsumoto Y, Furuhata Y, Nishihara M, Sasaki F, Takahashi M.
Obesity and insulin resistance in human growth hormone transgenic rats.
Endocrinology. 139(7):3057-63, 1998.

Furuhata Y, Kagaya R, Hirabayashi K, Ikeda A, Chang KT, Nishihara M, Takahashi M.
Development of obesity in transgenic rats with low circulating growth hormone levels: involvement of leptin resistance.
Eur J Endocrinol. 143(4):535-41, 2000.

Yamanouchi K, Yada E, Hozumi H, Ueno C, Nishihara M.
Analyses of hind leg skeletons in human growth hormone transgenic rats.
Exp Gerontol. 39(8):1179-88, 2004.

Hozumi H, Yamanouchi K, Nishihara M.
Involvement of neuropeptide Y in hyperphagia in human growth hormone transgenic rats.
J Vet Med Sci. 68(9):959-65, 2006.
Additional strain information