Strain information

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NBRP Rat No: 0547

Strain name: W-Tg(Prl-EGFP)Yamp

Commmon Name: Wistar-Tg(Prl-EGFP)Yamp, PRL-EGFP

Rat Genome Database

Principal Investigator:

Jun AritaGraduate School of Medical and Engneering, University of Yamanashi

Preservation Status:

Embryo Sperm Living Animals

Coat Color

albino (c)

Inbred Generations

Usage Restrictions

In publishing the research results to be obtained by use of the
BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(See references)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

Genetic Status

Inbred Segregating Congenic Consomic Recombinant Coisogenic Spont. Mutant Transgene Ind. Mutant Category Other

Comercial Availability

Research Category

Diabetes Obesity Neurobiology Ophthalmology Dentistry Cardio Hypertension Cancer Metabolism Otorhinology Immunology Infectious Osteosis Internal Organ Dermatology Reproduction Development Behavior Hematology Urology Pharmacology Research Area Others

Control Strain Marker Strain

Gene Affected

Origin

A transgenic construct was designed with the rat prolactin promoter (-3221 - 3233) controlling EGFP. Transgenic rats originated from Crlj:WI (Wistar) rats. (Jul 16, 2009)

Strain characteristics

In adenohypophysial cells, 55% cells are positive for GFP. Among GFP positive cells, 76% are immunostained with prolactin antibody. (Jul 16, 2009)

Breeding Conditions

The transgene has no effect on fertility. General breeding and reproduction rate. (Jul 16, 2009)

Genotyping

1. PCR analysis on genomic DNA. Primer sequences: Forward: 5'-CTGGCCACTATGTCTTCCTGA-3', Reverse: 5'-TGTAGTTGCCGTCGTCCTTGA-3'. Protocols: 95˚C-10 min, 35 cylcles of (94˚C-1 min, 64˚C-1 min, 72˚C-1 min) Product: about 860-bp. 2. Southern blotting with genomic DNA. EGFP + poly(A) (about 1-kb) is detected with EGFP cDNA probe on BamHI-cut genomic DNA. (Jul 16, 2009)

References

Ishida M, Takahashi W, Itoh S, Shimodaira S, Maeda S, Arita J.
Estrogen actions on lactotroph proliferation are independent of a paracrine interaction with other pituitary cell types: a study using lactotroph-enriched cells.
Endocrinology. 148(7):3131-9, 2007.

Additional strain information (Japanese only)