Strain information
 NBRP Rat No: 0554  Strain name: W-Tg(Slc32a1-YFP*)1Yyan  Commmon Name: Wistar-Tg(Slc32a1-YFP*)1Yyan, VGAT-Venus-A Rat Genome Database
Principal Investigator:  Yuchio Yanagawa  Gunma University Graduate School of Medicine       3-39-22 Showa-machi, Maebashi     371-8511 Gunma     Japan
Tel: 027-220-8040     Fax: 027-220-8046 Email: yuchio@gunma-u.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals ../images/Photos/W-TgYFP1/W-TgYFP1_1024.jpg ../images/Photos/
Coat Color  albino (c)
Inbred Generations  F?+9 (April 2012)
Usage Restrictions  1: The recipient of BIOLOGICAL RESOUCE shall obtain a prior written consent on use of it from the DEPOSITOR.

2: Recipient agrees to cite the following paper in all written publications as related to the Material.

Uematsu M, Hirai Y, Karube F, Ebihara S, Kato M, Abe K, Obata K, Yoshida S, Hirabayashi M, Yanagawa Y & Kawaguchi Y.
Quantitative chemical composition of cortical GABAergic neurons revealed in transgenic Venus-expressing rats.
Cereb Cortex 18, 315-330, 2008.

3: Recipient agrees to describe the following in acknowledgement in all written publications as related to the Materials.

“VGAT-Venus transgenic rats were generated by Drs. Y. Yanagawa, M. Hirabayashi and Y. Kawaguchi in National Institute for Physiological Sciences, Okazaki, Japan, using pCS2-Venus provided by Dr. A. Miyawaki.”


4: Recipient agrees to send the reprints of written publications as related to the Materials to Drs. Yasuo Kawaguchi and Yuchio Yanagawa, when they are published.

(Address)
Yuchio Yanagawa
Department of Genetic and Behavioral Neuroscience
Gunma University Graduate School of Medicine
3-39-22 Showa-machi, Maebashi 371-8511, Japan
E-mail: yuchio@gunma-u.ac.jp

Yasuo Kawaguchi
Division of Cerebral Circuitry
National Institute for Physiological Sciences
Myodaiji, Okazaki 444-8585, Japan
E-mail: yasuo@nips.ac.jp


5: The Venus gene incorporated into VGAT-Venus rats was supplied by Dr. Atsushi Miyawaki at RIKEN, Japan. The following is needed according to the Agreement.

Dr. Atsushi Miyawaki
Laboratory for Cell Function Dynamics
RIKEN Brain Science Institute
2-1 Hirosawa, Wako City
Saitama, 351-0198, JAPAN
Email: matsushi@brain.riken.jp

4-1: RECIPIENT agrees to submit the intended publication to Dr. Atsushi Miyawaki prior to the submission date.
4-2: RECIPIENT agrees to acknowledge Dr. Atsushi Miyawaki in all oral presentations and written publications as related to the MATERIAL. For example, “Venus was developed by Dr. Atsushi Miyawaki at RIKEN, Wako, Japan.”
4-3: RECIPIENT agrees to send the reprints in relation to all oral presentations or written publications as related to the Materials to Dr. Atsushi Miyawaki.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected YFP* (Venus): yellow fluorescent protein variant, Slc32a1: solute carrier family 32 member 1
Origin This transgenic rat was established at National Institute for Physiological Sciences in 2004, thereafter introduced to Gunma University in 2006. (Jun 22, 2009)
Strain characteristics This transgenic rat aims to establish Venus-labeled GABAergic neuron. There are 2 strains (A line: NBRP No.0554 and B line: NBRP No.0555) and expression and distribution of YFP* (Venus) are different from between the strains. In the frontal cortex, YFP* was expressed in 98% of GABAergic A line and 93% of that of B line. (Uematsu, 2007) (Jun 22, 2009)
Breeding Conditions Identify the transgenic rats by genotyping from progeny of crossing of hemizygous Tg rat and wild-type Wistar rat. (Jun 22, 2009)
Genotyping Genotyping protocol for Slc32a1-YFP*
References Degro CE, Kulik A, Booker SA, Vida I.
Compartmental distribution of GABAB receptor-mediated currents along the somatodendritic axis of hippocampal principal cells.
Front Synaptic Neurosci. 2015 Mar 23;7:6.


Ueta Y, Otsuka T, Morishima M, Ushimaru M, Kawaguchi Y.
Multiple layer 5 pyramidal cell subtypes relay cortical feedback from secondary to primary motor areas in rats.
Cereb Cortex. 2014 Sep;24(9):2362-76.

Otsuka T, Kawaguchi Y.
Common excitatory synaptic inputs to electrically connected cortical fast-spiking cell networks.
J Neurophysiol. 2013 Aug;110(4):795-806.

Uematsu M, Hirai Y, Karube F, Ebihara S, Kato M, Abe K, Obata K, Yoshida S, Hirabayashi M, Yanagawa Y & Kawaguchi Y.
Quantitative chemical composition of cortical GABAergic neurons revealed in transgenic Venus-expressing rats.
Cereb Cortex 18, 315-330, 2008.
Additional strain information