| NBRP Rat No: 0576 |
Strain name: ALD/Hyo |
Commmon Name: Wolman's disease rat, Yoshida rat |
Rat Genome Database |
| Principal Investigator: |
Hiroki Yoshida Kagoshima University 1-21-24 Korimoto Kagoshima 890-8580 Kagoshima Japan |
| Tel: 099-285-7000 Fax: 099-285-7001 |
Email: gakucyo@kuas.kagoshima-u.ac.jp |
| Preservation Status: |
Embryo Sperm Living Animals |
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| Coat Color |
albino (c) |
| Inbred Generations |
F40+? (May 2009) |
| Usage Restrictions |
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| Genetic Status |
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| Comercial Availability |
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| Research Category |
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| Gene Affected |
Lipa: lipase A, lysosomal acid, cholesterol esterase |
| Origin |
In a colony of Donryu rats, Yoshida found a male rat showing hepatomegaly and splenomegaly in 1981 (Yoshida, 1990). These mutant rats were transferred to Kagoshima University and maintained in heterozygous condition so far. ALD (acid lipase deficiency). (Mar 15, 2010) |
| Strain characteristics |
ALD rats have congenital deficiency of lysosomal acid lipase. Affected rats markedly accumulate cholesteryl ester, free cholesterol and triglyceride in the liver, and cholesteryl esters and free cholesterol in the spleen (Kuriyama, 1990; Kuriwaki, 1999). ALD is a disease model for Wolman’s disease.
ALD rat has a large deletion at the 3’-end of the Lipa gene (Nakagawa, 1995). (Mar 15, 2010) |
| Breeding Conditions |
Homozygous animals die until 120 days of age from liver failures. Homozygous ALD rats are infertile. Maintained in heterozygous condition. (Mar 15, 2010) |
| Genotyping |
Please refer to reference 3 (Nakagawa, 1995).
Genotyping protocol for Lich |
| References |
1.Yoshida H, Kuriyama M.
Genetic lipid storage disease with lysosomal acid lipase deficiency in rats.
Lab Anim Sci. 1990 Sep;40(5):486-9.
2.Kuriyama M, Yoshida H, Suzuki M, Fujiyama J, Igata A.
Lysosomal acid lipase deficiency in rats: lipid analyses and lipase activities in liver and spleen
J Lipid Res. 1990 Sep;31(9):1605-12.
3.Nakagawa H, Matsubara S, Kuriyama M, Yoshidome H, Fujiyama J, Yoshida H, Osame M.
Cloning of rat lysosomal acid lipase cDNA and identification of the mutation in the rat model of Wolman's disease.
J Lipid Res. 1995 Oct;36(10):2212-8.
4.Kuriwaki K, Yoshida H.
Morphological characteristics of lipid accumulation in liver-constituting cells of acid lipase deficiency rats (Wolman's disease model rats).
Pathol Int. 1999 Apr;49(4):291-7.
5. Thelwall PE, Smith FE, Leavitt MC, Canty D, Hu W, Hollingsworth KG, Thoma C, Trenell MI, Taylor R, Rutkowski JV, Blamire AM, Quinn AG.
Hepatic Cholesteryl Ester Accumulation in Lysosomal Acid Lipase Deficiency: Non-invasive Identification and Treatment Monitoring by Magnetic Resonance.
J Hepatol. 2013 Sep;59(3):543-9. |
| Additional strain information |
ALD |
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