| NBRP Rat No: 0604 |
Strain name: W-Tg(Tek-GFP)1Soh |
Commmon Name: Tie2-GFP |
Rat Genome Database |
| Principal Investigator: |
Tetsuya Terasaki Tohoku University 6-3 Aoba Aramaki aza Aoba-ku, Sendai 980-8578 Miyagi Japan |
| Tel: 022-795-6832 Fax: 022-795-6886 |
Email: sohtsuki@mail.pharm.tohoku.ac.jp |
| Preservation Status: |
Embryo Sperm Living Animals |
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| Coat Color |
albino (c) |
| Inbred Generations |
F2-3 (Jun 2010) |
| Usage Restrictions |
In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested and an acknowledgment to the DEPOSITOR is also requested.
Ohtsuki S. et al., Vascular endothelium-selective gene induction by Tie2 promoter/enhancer in the brain and retina of a transgenic rat. Pharm Res 2005 Jun;22(6):852-7. |
| Genetic Status |
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| Comercial Availability |
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| Research Category |
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| Gene Affected |
Tie2 promoter-GFP (Original plasmid name; pSP14/15.t2hgfpPan5’) |
| Origin |
This transgenic strain was generated by microinjection into fertilized oocytes of Wistar rats (Ohtsuki, 2005). The Tek/GFP plasmid (pSP14/15.t2hgfpPan5’) was a given from Dr. TN Sato of University of Texas Southwestern Medical Center at Dallas (Motoike 2000). The transgene was excised from the plasmid vector by Sal I digestion. (Aug 31, 2010) |
| Strain characteristics |
GFP is expressed in capillary endothelial cells under the control of Tek (other name is Tie2) promoter. The blood vessel is visible in this strain. (Aug 31, 2010) |
| Breeding Conditions |
normal breeding |
| Genotyping |
Genotyping by PCR analysis.
Extract-N-Amp Tissue PCR kit (SIGMA) Primer 1: 5’-ATTCTCGTGGAACTGGATGG-3’ Primer 2: 5’-GGACAGGTAATGGTTGTCTGG-3’ Signal: 567 bp Cycle Program: 1 cycle of 94C for 3 min, 40 cycles of 94C for 30 s, 55C for 1 min, 72C for 1 min, and a final elongation of 72 C for 2 min. (Aug 31, 2010) |
| References |
Koseki H, Miyata H, Shimo S, Ohno N, Mifune K, Shimano K, Yamamoto K, Nozaki K, Kasuya H, Narumiya S, Aoki T.
Two Diverse Hemodynamic Forces, a Mechanical Stretch and a High Wall Shear Stress, Determine Intracranial Aneurysm Formation.
Transl Stroke Res. 2020 Feb;11(1):80-92.
Ohtsuki S, Kamiya N, Hori S, Terasaki T.
Vascular endothelium-selective gene induction by Tie2 promoter/enhancer in the brain and retina of a transgenic rat.
Pharm Res. 2005 Jun;22(6):852-7. Epub 2005 Jun 8.
Motoike T, Loughna S, Perens E, Roman BL, Liao W, Chau TC, Richardson CD, Kawate T, Kuno J, Weinstein BM, Stainier DY, Sato TN.
Universal GFP reporter for the study of vascular development.
Genesis. 2000 Oct;28(2):75-81.
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| Additional strain information |
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