Strain information
 NBRP Rat No: 0604  Strain name: W-Tg(Tek-GFP)1Soh  Commmon Name: Tie2-GFP Rat Genome Database
Principal Investigator:  Tetsuya Terasaki  Tohoku University       6-3 Aoba Aramaki aza Aoba-ku, Sendai    980-8578 Miyagi     Japan
Tel: 022-795-6832    Fax: 022-795-6886 Email: sohtsuki@mail.pharm.tohoku.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals ../images/Photos/ ../images/Photos/
Coat Color  albino (c)
Inbred Generations  F2-3 (Jun 2010)
Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested and an acknowledgment to the DEPOSITOR is also requested.
Ohtsuki S. et al., Vascular endothelium-selective gene induction by Tie2 promoter/enhancer in the brain and retina of a transgenic rat. Pharm Res 2005 Jun;22(6):852-7.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 組織学
 Control Strain  Marker Strain
Gene Affected Tie2 promoter-GFP (Original plasmid name; pSP14/15.t2hgfpPan5’)
Origin This transgenic strain was generated by microinjection into fertilized oocytes of Wistar rats (Ohtsuki, 2005). The Tek/GFP plasmid (pSP14/15.t2hgfpPan5’) was a given from Dr. TN Sato of University of Texas Southwestern Medical Center at Dallas (Motoike 2000). The transgene was excised from the plasmid vector by Sal I digestion. (Aug 31, 2010)
Strain characteristics GFP is expressed in capillary endothelial cells under the control of Tek (other name is Tie2) promoter. The blood vessel is visible in this strain. (Aug 31, 2010)
Breeding Conditions normal breeding
Genotyping Genotyping by PCR analysis.
Extract-N-Amp Tissue PCR kit (SIGMA) Primer 1: 5’-ATTCTCGTGGAACTGGATGG-3’ Primer 2: 5’-GGACAGGTAATGGTTGTCTGG-3’ Signal: 567 bp Cycle Program: 1 cycle of 94C for 3 min, 40 cycles of 94C for 30 s, 55C for 1 min, 72C for 1 min, and a final elongation of 72 C for 2 min. (Aug 31, 2010)
References Motoike T, Loughna S, Perens E, Roman BL, Liao W, Chau TC, Richardson CD, Kawate T, Kuno J, Weinstein BM, Stainier DY, Sato TN.
Universal GFP reporter for the study of vascular development.
Genesis. 2000 Oct;28(2):75-81.

Ohtsuki S, Kamiya N, Hori S, Terasaki T.
Vascular endothelium-selective gene induction by Tie2 promoter/enhancer in the brain and retina of a transgenic rat.
Pharm Res. 2005 Jun;22(6):852-7. Epub 2005 Jun 8.
Additional strain information