Strain information
NBRP Rat No: 0585
Strain name
: F344-
Il2rg
em1Kyo
Commmon Name: X-SCID
Rat Genome Database
Principal Investigator:
Tomoji Mashimo The University of Tokyo, The Institute of Medical Science 4-6-1 Shirokanedai-Minatoku-Tokyoto 108-8639 Japan
Tel: 03-6409-2489 Fax:
Email: mashimo@ims.u-tokyo.ac.jp
Preservation Status:
Embryo
Sperm
Living Animals
Coat Color
albino (c)
Inbred Generations
N3
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The recipient must obey regulations stipulated in the liscence agreement of Sigma-Aldrich Co. LLC.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Category Other
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio Hypertension
Cancer
Metabolism
Otorhinology
Immunology
Infectious
Osteosis
Internal Organ
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Research Area Others
Control Strain
Marker Strain
Gene Affected
interleukin 2 receptor gamma (Il2rg)
Origin
This strain is established by ZFN method targeting rat Il2rg gene. Background strain: F344/Stm
Strain characteristics
This strain shows severe combined immunodeficiency caused by a 660 bp deletion in Il2rg gene and grow normally under SPF condition.
Breeding Conditions
maintained by mating between hemizygous male and homozygous female
Genotyping
Genotyping by PCR analysis. wild：1509-bp, mutant：849-bp primers：TCTCCCAGGGGACTTAGCTTA, TGGGACCTGGTGTTAGGTTT
References
Mashimo T, Takizawa A, Voigt B, Yoshimi K, Hiai H, Kuramoto T, Serikawa T.
Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases.
PLoS One. 2010 Jan 25;5(1):e8870.
Additional strain information