Strain information
 NBRP Rat No: 0586  Strain name: F344-Il2rgem2Kyo  Commmon Name: X-SCID Rat Genome Database
Principal Investigator:  Tomoji Mashimo  The University of Tokyo, The Institute of Medical Science       4-6-1 Shirokanedai-Minatoku-Tokyoto    108-8639      Japan
Tel: 03-6409-2489    Fax:  Email:
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  
Inbred Generations  N1F9 (April 2012)
Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The recipient must obey regulations stipulated in the liscence agreement of Sigma-Aldrich Co. LLC.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected interleukin 2 receptor gamma (Il2rg)
Origin This strain is established by ZFN method targeting rat Il2rg gene. Background strain: F344/Stm
Strain characteristics This strain shows severe combined immunodeficiency caused by a 1097-bp deletion in Il2rg gene and grow normally under SPF condition.
Breeding Conditions maintained by mating between hemizygous male and homozygous female
Genotyping primers: TCTCCCAGGGGACTTAGCTTA, TGGGACCTGGTGTTAGGTTT. product size: wild 1509-bp, mutant 412-bp
References Ohno T, Kai T, Miyasaka Y, Maruyama H, Ishih A, Kino H.
Intestinal immunity suppresses carrying capacity of rats for the model tapeworm, Hymenolepis diminuta.
Parasitol Int. 2018 Feb 12. pii: S1383-5769(18)30004-7.

Katsukawa M, Nakajima Y, Fukumoto A, Doi D, Takahashi J.
Fail-safe therapy by gamma-ray irradiation against tumor formation by human induced pluripotent stem cell-derived neural progenitors.
Stem Cells Dev. 2016 Jun 1;25(11):815-25.

Nishimura K, Doi D, Samata B, Murayama S, Tahara T, Onoe H, Takahashi J.
Estradiol Facilitates Functional Integration of iPSC-Derived Dopaminergic Neurons into Striatal Neuronal Circuits via Activation of Integrin α5β1.
Stem Cell Reports. 2016 Apr 12;6(4):511-24.

Yamashita, A., Morioka, M., Yahara, Y., Okada, M., Kobayashi, T., Kuriyama, S., Matsuda, S., and Tsumaki, N.
Generation of Scaffoldless Hyaline Cartilaginous Tissue from Human iPSCs.
Stem Cell Reports. 2015 Mar 10;4(3):404-418

Samata B, Kikuchi T, Miyawaki Y, Morizane A, Mashimo T, Nakagawa M, Okita K, Takahashi J.
X-linked severe combined immunodeficiency (X-SCID) rats for xeno-transplantation and behavioral evaluation.
J Neurosci Methods. 243:68-77, 2015.

Mashimo T, Takizawa A, Kobayashi J, Kunihiro Y, Yoshimi K, Ishida S, Tanabe K, Yanagi A, Tachibana A, Hirose J, Yomoda J, Morimoto S, Kuramoto T, Voigt B, Watanabe T, Hiai H, Tateno C, Komatsu K, Serikawa T.
Generation and characterization of severe combined immunodeficiency rats.
Cell Rep. 2012 Sep 27;2(3):685-94.

Mashimo T, Takizawa A, Voigt B, Yoshimi K, Hiai H, Kuramoto T, Serikawa T.
Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases.
PLoS One. 2010 Jan 25;5(1):e8870.
Additional strain information