Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The recipient must obey regulations stipulated in the liscence agreement of Sigma-Aldrich Co. LLC.
Gene Affected
interleukin 2 receptor gamma (Il2rg)
Origin
This strain is established by ZFN method targeting rat Il2rg gene. Background strain: F344/Stm
Strain characteristics
This strain shows severe combined immunodeficiency caused by a 1097-bp deletion in Il2rg gene and grow normally under SPF condition.
Breeding Conditions
maintained by mating between hemizygous male and homozygous female
Genotyping
primers: TCTCCCAGGGGACTTAGCTTA, TGGGACCTGGTGTTAGGTTT. product size: wild 1509-bp, mutant 412-bp
References
Mizuno-Iijima S, Kawamoto S, Asano M, Mashimo T, Wakana S, Nakamura K, Nishijima KI, Okamoto H, Saito K, Yoshina S, Miwa Y, Nakamura Y, Ohkuma M, Yoshiki A.
Mammalian genome research resources available from the National BioResource Project in Japan.
Mamm Genome. 2024 Dec;35(4):497-523.
Miyawaki Y, Samata B, Kikuchi T, Nishimura K, Takahashi J.
Zonisamide promotes survival of human-induced pluripotent stem cell-derived dopaminergic neurons in the striatum of female rats.
J Neurosci Res. 2020 Aug;98(8):1575-1587.
Ohno T, Kai T, Miyasaka Y, Maruyama H, Ishih A, Kino H.
Intestinal immunity suppresses carrying capacity of rats for the model tapeworm, Hymenolepis diminuta.
Parasitol Int. 2018 Aug;67(4):357-361.
Katsukawa M, Nakajima Y, Fukumoto A, Doi D, Takahashi J.
Fail-safe therapy by gamma-ray irradiation against tumor formation by human induced pluripotent stem cell-derived neural progenitors.
Stem Cells Dev. 2016 Jun 1;25(11):815-25.
Nishimura K, Doi D, Samata B, Murayama S, Tahara T, Onoe H, Takahashi J.
Estradiol Facilitates Functional Integration of iPSC-Derived Dopaminergic Neurons into Striatal Neuronal Circuits via Activation of Integrin α5β1.
Stem Cell Reports. 2016 Apr 12;6(4):511-24.
Yamashita, A., Morioka, M., Yahara, Y., Okada, M., Kobayashi, T., Kuriyama, S., Matsuda, S., and Tsumaki, N.
Generation of Scaffoldless Hyaline Cartilaginous Tissue from Human iPSCs.
Stem Cell Reports. 2015 Mar 10;4(3):404-418
Samata B, Kikuchi T, Miyawaki Y, Morizane A, Mashimo T, Nakagawa M, Okita K, Takahashi J.
X-linked severe combined immunodeficiency (X-SCID) rats for xeno-transplantation and behavioral evaluation.
J Neurosci Methods. 243:68-77, 2015.
Mashimo T, Takizawa A, Kobayashi J, Kunihiro Y, Yoshimi K, Ishida S, Tanabe K, Yanagi A, Tachibana A, Hirose J, Yomoda J, Morimoto S, Kuramoto T, Voigt B, Watanabe T, Hiai H, Tateno C, Komatsu K, Serikawa T.
Generation and characterization of severe combined immunodeficiency rats.
Cell Rep. 2012 Sep 27;2(3):685-94.
Mashimo T, Takizawa A, Voigt B, Yoshimi K, Hiai H, Kuramoto T, Serikawa T.
Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases.
PLoS One. 2010 Jan 25;5(1):e8870.