Strain information  NBRP Rat No: 0651  Strain name: WIAR.MPR-Arsbabd/Ncchd  Commmon Name: MPR Rat Genome Database Principal Investigator:  XIAO-KANG LI  National Research Institute for Child Health and Development       2-10-1, Okura, Setagaya-ku,     157-8535 Tokyo     ＪＡＰＡＮ Tel: 03-5494-8283    Fax: 03-415-2171 Email:  ri-k@ncchd.go.jp Preservation Status:   Embryo        Sperm       Living Animals Coat Color  白 Inbred Generations   Usage Restrictions   Genetic Status   Inbred  Segregating   Congenic   Consomic   Recombinant   Coisogenic   Spont. Mutant  Transgene  Ind. Mutant  Category Other  Comercial Availability Research Category   Diabetes Obesity  Neurobiology  Ophthalmology   Dentistry  Cardio Hypertension  Cancer  Metabolism  Otorhinology  Immunology  Infectious  Osteosis   Internal Organ  Dermatology   Reproduction   Development  Behavior  Hematology  Urology   Pharmacology  Research Area Others   Control Strain  Marker Strain Gene Affected Arsb: arylsulfatase B Origin This congenic strain was established by backcrossing MPR/Iar (provided by Dr. Kunieda, Okayama University) to WIAR/Iar Strain characteristics This strain does not show organ rejection reaction after organ transplantation. Under analysis for detailed phenotype. Breeding Conditions Maintained as segregating inbred strain (Arsb is heterozygous). Arsb-homozygous rats (both male and female) are unsuitable for breeding. Genotyping Sequence analysis to detect one-base insertion of C, resulting in right contiguous "C" from nucleotide 664 in the exon3. Primers: Primer F (GCGAAGAACCTGAAAAGAG) and Primer R (TGCACACACACACACACACA) PCR condition：80℃ 15min, 94℃ 5min, (94℃0.5min,60℃ 1min,72℃ 1min)×35cycle, 72℃ 7min. References Yoshida M, Noguchi J, Ikadai H, Takahashi M, Nagase S. Arylsulfatase B-deficient mucopolysaccharidosis in rats. J Clin Invest. 91(3):1099-104, 1993. Kunieda T, Simonaro CM, Yoshida M, Ikadai H, Levan G, Desnick RJ, Schuchman EH. Mucopolysaccharidosis type VI in rats: isolation of cDNAs encoding arylsulfatase B, chromosomal localization of the gene, and identification of the mutation. Genomics. 10;29(3):582-7, 1995. 国枝哲夫,ムコ多糖症Ⅵ型ラットの遺伝子治療のためのモデル動物としての確立,1997 Toyama S, Migita O, Fujino M, Kunieda T, Kosuga M, Fukuhara Y, Nagahara Y, Li XK, Okuyama T. Liver transplantation: New treatment for mucopolysaccharidosis type VI in rats. Pediatr Int. 2019 Feb;61(2):180-189. doi: 10.1111/ped.13751. Epub 2019 Feb 21. Additional strain information