Japanese
 NBRP Rat No: 0283 Strain NameW-Tg(CAG-cre)81Jmsk Commmon Name: Wistar-Tg(CAG-cre)81Jmsk, Wistar-<i>Tg(CAG-NCre)81Jmsk</i>,&nbsp;NCre81
 Principal Investigator  Eiji Kobayashi
 Organization   Center for Molecular Medicine, Jichi Medical School Division of Organ Replacement Research
 Address  3311-1, Yakushiji, Shimono-shi, Tochigi

329-0498 Tochigi

 Japan
 Telephone  0285-58-7446  Fax:  0285-44-5365  eijikoba@jichi.ac.jp
 Inbred Generations   F?+21 (March 2012) 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  The recipient is requested to cite in the following literature designated by the DEPOSITOR. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene
 Origin This strain was established at Jichi Medical School. 
 Strain Characteristics This strain expresses the Cre recombinase ubiquitously driven by CAG promoter. The majority of expression of the transgene is detected in the skeletal muscles. Transgene was integrated into the Chr X. 
 Breeding Conditions Good breeding performance. 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/CAG Promoter_en.pdf">Genotyping protocol for CAG Promoter</a>
<a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/Cre_en.pdf">Genotyping protocol for Cre</a> 
 References  Fukamachi K, Tanaka H, Sakai Y, Alexander DB, Futakuchi M, Tsuda H, Suzui M.
A novel reporter rat strain that expresses LacZ upon Cre‚Äźmediated recombination.
Genesis. 2013 Apr;51(4):268-74.

Sato Y, Endo H, Ajiki T, Hakamata Y, Okada T, Murakami T, Kobayashi E.
Establishment of Cre/LoxP recombination system in transgenic rats.
Biochem Biophys Res Commun. 9;319(4):1197-202, 2004.