Japanese
 NBRP Rat No: 0297 Strain NameLEW-Tg(CAG-EGFP)1Ys Commmon Name: LEW-<i>TgN(CAG-EGFP)1Ys</i>, GFP LEW
 Principal Investigator  Eiji Kobayashi
 Organization   Center for Molecular Medicine, Jichi Medical School Division of Organ Replacement Research
 Address  3311-1, Yakushiji, Shimono-shi, Tochigi

329-0498 Tochigi

 Japan
 Telephone  0285-58-7446  Fax:  0285-44-5365  eijikoba@jichi.ac.jp
 Inbred Generations   F18 (March 2012) 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions   
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene GFP: Green Fluorescent Protein, jellyfish
 Origin This transgenic strain was established at YS institute. 
 Strain Characteristics This transgenic strain contains the enhanced green fluorescent protein (EGFP) gene ubiquitously driven by CAG promoter. 
 Breeding Conditions Good breeding performance. 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/CAG Promoter_en.pdf">Genotyping protocol for CAG Promoter</a>
<a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/EGFP_en.pdf">Genotyping protocol for EGFP</a> 
 References  Esmaeili R, Sadeghpour A, Darbandi-Azar A, Majidzadeh-A K, Vajhi A, Sadeghizadeh M
Echocardiographic assessment of myocardial infarction: comparison of a rat model in two strains.
Iran J Vet Res. 2017 Winter;18(1):30-35.

Kaizawa Y, Kakinoki R, Ikeguchi R, Ohta S, Noguchi T, Takeuchi H, Oda H, Yurie H, Matsuda S.
A Nerve Conduit Containing a Vascular Bundle and Implanted With Bone Marrow Stromal Cells and Decellularized Allogenic Nerve Matrix.
Cell Transplant. 2017 Feb 16;26(2):215-228.

Maeda H, Okamoto K, Namikawa T, Tsuda M, Uemura S, Shiga M, Hanazaki K, Kobayashi M.
Re-evaluation of hepatocyte replacement by recipient-derived cells after allogenic liver transplantation: Discrepancy between clinical observations and a rat model.
Hepatol Res. 2016 Jan 4. [Epub ahead of print]

Tomita K, Nishibayashi A, Yano K, Hosokawa K.
Adipose-derived stem cells protect against endoneurial cell death: Cell therapy for nerve autografts.
Microsurgery. 2015 Jul 20 [Epub ahead of print]

Ohshima M, Taguchi A, Tsuda H, Sato Y, Yamahara K, Harada-Shiba M, Miyazato M, Ikeda T, Iida H, Tsuji M.
Intraperitoneal and intravenous deliveries are not comparable in terms of drug efficacy and cell distribution in neonatal mice with hypoxia-ischemia.
Brain Dev. 2015 Apr;37(4):376-386, 2015.

Tomita Koichi, Nishibayashi Akimitsu, Yano Kenji, Hosokawa Ko.
Differentiated Adipose-derived Stem Cells Promote Reinnervation of Rat Skin Flaps.
Plast Reconstr Surg Glob Open 2013;1:e22

Ishikane S, Yamahara K, Sada M, Harada K, Kodama M, Ishibashi-Ueda H, Hayakawa K, Mishima K, Iwasaki K, Fujiwara M, Kangawa K, Ikeda T.
Allogeneic administration of fetal membrane-derived mesenchymal stem cells attenuates acute myocarditis in rats.
J Mol Cell Cardiol. 2010 Nov;49(5):753-61.

Tsuda H, Yamahara K, Ishikane S, Otani K, Nakamura A, Sawai K, Ichimaru N, Sada M, Taguchi A, Hosoda H, Tsuji M, Kawachi H, Horio M, Isaka Y, Kangawa K, Takahara S, Ikeda T.
Allogenic fetal membrane-derived mesenchymal stem cells contribute to renal repair in experimental glomerulonephritis.
Am J Physiol Renal Physiol. 2010 Nov;299(5):F1004-13.

Inoue H, Ohsawa I, Murakami T, Kimura A, Hakamata Y, Sato Y, Kaneko T, Takahashi M, Okada T, Ozawa K, Francis J, Leone P, Kobayashi E.
Development of new inbred transgenic strains of rats with LacZ or GFP.
Biochem Biophys Res Commun. 329(1):288-295, 2005.