NBRP Rat No: 0469 Strain NameW-Tg(Gnrh1-EGFP)Nphy Commmon Name: Wistar-Tg(Gnrh1-EGFP)Nphy, Wistar-<i>Tg(GnRH-EGFP)Nphy</i>
 Principal Investigator  Masakatsu Kato
 Organization   Nippon Medical school Department of Physiology and Behavioral Neuroendocrinology
 Address  1-1-5, Sendagi, Bunkyo-ku

113-8602 Tokyo

 Telephone  03-3822-2131 ext 5242  Fax:  03-5685-3055  mkato@nms.ac.jp
 Inbred Generations   F?+13 (April 2012) 
 Coat Color
 Deposition Status
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions   In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested. (see References) 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene GFP: Green Fluorescent Protein, jellyfish
 Origin This trans genic strain was established in 2001. 
 Strain Characteristics This transgenic strain contains the enhanced green fluorescent protein (EGFP) gene driven by gonadotropin-releasing hormone 1 (Gnrh1) promoter. EGFP fluorescence is observed only in Gnrh1-immunoreactive neurons, approximately one third of which has strong EGFP fluorescence. 
 Breeding Conditions Since high inbred generations reduce breeding performance, crossing to wild type is necessary once in a while. 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/Gnrh1-EGFP_en.pdf">Genotyping protocol for Gnrh1-EGFP</a>
 References  Kato M, Ui-Tei K, Watanabe M, Sakuma Y.
Characterization of voltage-gated calcium currents in gonadotropin-releasing hormone neurons tagged with green fluorescent protein in rats.
Endocrinology. 144(11):5118-25, 2003.

Yin C., Ishii H., Tanaka N., Sakuma Y., Kato M.
Activation of A-type g-amino butyric acid receptors (GABAARs) excites gonadotropin-releasing hormone (GnRH) neurones isolated from adult rats.
J Neuroendocrinol. 2008 May;20(5):566-75.

Kato M, Tanaka N, Usui S, Sakuma Y.
The SK channel blocker apamin inhibits slow afterhyperpolarization currents in rat gonadotropin-releasing hormone neurones.
J Physiol. 15;574(Pt 2):431-42, 2006.

Watanabe M., Sakuma Y., Kato M.
GABAA receptors mediated excitation in adult rat GnRH neurons.
Biol Reprod. 2009 Aug;81(2):327-332.

Kato M., Tanaka N., Ishii H., Yin C., Sakuma Y.
Ca2+ channels and Ca2+-activated K+ channels in adult rat gonadotrophin-releasing hormone neurones.
J Neuroendocrinol. 2009 Mar;21(4):312-315.

Tanaka N., Ishii H., Yin C., Koyama M., Sakuma Y., Kato M.
Voltage-gated Ca2+ channel mRNAs and T-type Ca2+ currents in rat gonadotropin-releasing hormone neurons.
J. Physiol. Sci. 2010 May;60(3):195-204.

Koyama M, Yin C, Ishii H, Sakuma Y, Kato M.
Somatostatin inhibition of GnRH neuronal activity and the morphological relationship between GnRH and somatostatin neurons in rats.
Endocrinology. 2012 Feb;153(2):806-814.

Tada H, Kuroki Y, Funabashi T, Kamiya Y, Goto T, Suyama K, Sano A, Mitsushima D, Etgen AM, Takahashi T.
Phasic synaptic incorporation of GluR2-lacking AMPA receptors at gonadotropin-releasing hormone neurons is involved in the generation of the luteinizing hormone surge in female rats.
Neuroscience. 2013 Sep 17;248:664-66