Strain information
 NBRP Rat No: 0717  Strain name: LEA-Tg(Pou5f1-YFP*)3Ncco  Commmon Name: Oct4-Venus Tg rat, LEA-esTgN(Oct4/Venus)3NCCRI Rat Genome Database
Principal Investigator:  Takahiro Ochiya  Tokyo Medical University Institute of Medical Science        6-7-1 Nishi-Shinjuku, Shinjyuku-ku,     160-0023 Tokyo      Japan
Tel: 03-3342-6111    Fax:  Email: tochiya@tokyo-med.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals
Coat Color  茶色
Inbred Generations  不明
Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The recipient is requested to cite the literature designated by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others 
 Control Strain  Marker Strain
Gene Affected Oct4 (Pou5f1: POU class 5 homeobox 1 ) promoter, Venus (YFP*: Improved version of yellow fluorescent protein)
Origin This strain was provided from Kyoto Bioresource center.
Strain characteristics Venus fluorescence can be detected in Oct4(Pou5f1)-expressing cells (especially in germ cell and ES cells)
Breeding Conditions Mating between Tg rat and wild-type LEA rat.
Genotyping PCR analysis, 200-bp Forward: ACCCTGAAGCTGATCTGCAC Reverse: GGTCTTGTAGTTGCCGTCGT KOD version2 94℃: 2 min 98℃: 20 sec 62℃: 20 sec 68℃: 30 sec (35 cysles) 68℃: 5 min 4℃
References Kawamata M, Ochiya T. Generation of genetically modified rats from embryonic stem cells. Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14223-8.

Kawamata M, Ochiya T. Two distinct knockout approaches highlight a critical role for p53 in rat development. Sci Rep 2012 2: 945
Additional strain information