Strain information
 NBRP Rat No: 0489  Strain name: F344-Tg(CAG-EGFP)Ncco  Commmon Name: F344/DuCrlClrj-Tg(CAG-EGFP)Ncco, F344/DuCrlCrlj-TgN(CAG-EGFP)Ncco, F344-EGFP rat Rat Genome Database
Principal Investigator:  Takahiro Ochiya  Tokyo Medical University Institute of Medical Science        6-7-1 Nishi-Shinjuku, Shinjyuku-ku,     160-0023 Tokyo      Japan
Tel: 03-3342-6111    Fax:  Email: tochiya@tokyo-med.ac.jp
Preservation Status:   Embryo        Sperm       Living Animals ../images/Photos/F344_TgCAG_EGFP_Ncco/F344_TgCAG_EGFP_Ncco_1024.jpg ../images/Photos/F344_TgCAG_EGFP_Ncco/F344_TgCAG_EGFP_Ncco_GFP_1024.jpg
Coat Color  albino (c)
Inbred Generations  F4+14 (March 2012)
Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing, an acknowledgment to the DEPOSITOR is requested.
For a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from the DEPOSITOR.
Genetic Status
 Inbred  Segregating  Congenic  Consomic  Recombinant
 Coisogenic  Spont. Mutant  Transgene  Ind. Mutant  Category Other 
Comercial Availability
Research Category
 Diabetes Obesity  Neurobiology  Ophthalmology  Dentistry  Cardio Hypertension
 Cancer  Metabolism  Otorhinology  Immunology  Infectious
 Osteosis  Internal Organ  Dermatology  Reproduction  Development
 Behavior  Hematology  Urology  Pharmacology  Research Area Others Transplantation
 Control Strain  Marker Strain
Gene Affected GFP: Green Fluorescent Protein, jellyfish
Origin Transgenic rat: CAG promoter, Enhanced Green Fluorescent Protein gene, microinjection method
Strain characteristics Fluorescence is detected from almost whole tissues; skin, brain, heart, lung, spleen, liver, kidney, testis, sperm, uterus, muscle.
Breeding Conditions This strain is maintained by natural mating of Tg rats showing fluorescence with F344Du/Crj.
Genotyping Tg rats are selected by checking green fluorescence. Genotyping protocol for EGFP
References Zhao Q, Ogino S, Sowa Y, Lee S, Kato Y, Li Y, Sakamoto M, Yamanaka H, Nakano T, Sawaragi E, Tabata Y, Morimoto N.
A Bioabsorbable Implant Seeded with Adipose-Derived Stem Cells for Adipose Regeneration.
Tissue Eng Part A. 2025 Aug;31(15-16):1051-1065.

Watanabe H, Ochiya T, Ueda S, Kominami Y, Gon R, Nishiki M, Hayashi M, Sasaki A, Shiraishi M, Kashimoto N, Myojin Y, Kamiya K.
Differentiation of a hepatic phenotype after heterotropic transplantation of heart, kidney, brain, and skin tissues into liver in F344 rats.
Biochem Biophys Res Commun. 2007 Mar 23;354(4):841-5.
Additional strain information F344