Japanese
 NBRP Rat No: 0657 Strain Name: W-Tg(Per1-luc)Ylab Commmon Name: Per1-lucWistar
 Principal Investigator  Shin Yamazaki
 Organization   Vanderbilt University  Department of Biological Sciences
 Address   Room U8231A, MRBIII, 465 21st Ave South

37232   Nashsville, TN

 USA
 Telephone  USA (615)-322-8054  Fax:  USA (615) 343-6707  shin.yamazaki@vanderbilt.edu
 Inbred Generations   Crl:WI (stock#003, CharlesRiver USA), N16 (May 10/2011) 
   
 Coat Color
 Deposition Status
 
 Albino
  Embryo      Sperm      Live Animals
 Usage Restrictions  1:Use of this biological resource in the commercial enterprises is not permitted in principle.
2:Use of this biological resource by Companies or for-profit entities requires a license from University of Virginia Patent Foundation prior to shipping.

CONTACT FOR LICENSE INQUIRIES:
Stephanie A. Miller, Ph.D.
Licensing Associate
UVA Patent Foundation
250 W. Main St. Suite 300
Charlottesville, VA 22902
Phone: 434-982-1608
Email: stephanie@uvapf.org
 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene mouse Period1 プロモーターが、Luciferase 発光レポーターを駆動する外来遺伝子をもつトランスジェニックラット
 Origin The original work using this transgenic rat was published in Science (Yamazaki et al. 2000). This transgenic rat was generated in the Wistar outbred strain (Charles River, Japan). We maintained the L1 line. The transgenic rat was generated using a DNA construct in which the mouse Period1 promoter drives firefly luciferase (Hida et al, 2000). 
 Strain Characteristics The circadian rhythm in Per1 transcription is easily monitored from tissue explants (Yamazaki and Takahashi 2005). 
 Breeding Conditions Good breeding performance, similar to the Wistar strain. 
 Genotyping Measuring luminescence from a tail snip is the best, most reliable, and easiest way to determine genotype. Aliquot ~20μl of a solution containing 0.1mM to 1mM beetle luciferin in water into eppendorf tubes. Measure the photon counts in tube containing only the luciferin solution as a black (usually around 50 cps). Place a freshly cut tail tip in the eppendorf tube (cut end submerged in solution) and measure the photon counts (over 10,000 cps).
PCR genotype can also be performed using the following conditions:
The transgene can be identified by PCR using the two primer pairs within the luciferase gene.
PCR was performed at
95 °C (30 sec) for 1cycle,
95 °C (30 sec), 57 °C (30 sec),　72 °C (60 sec) for 30 cycles and
72 °C (60 sec) for 1 cycle.
Primer sequences are:
Luc F:TTTATAATGAACGTGAATTGCTC (323-345 of luciferase ORF)
and Luc R:CGTATTTGTCAATCAGAGTGC (893-913of luciferase ORF ) .
The size of PCR product is 590bp.
 
 References  <Full description of DNA construct used to generate this transgenic rat>
Hida A, Koike N, Hirose M, Hattori M, Sakaki Y, Tei H (2000) The human and mouse Period1 genes: five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription. Genomics 65:224-233.

<Detailed methods for luminescence recording>
Yamazaki S, Takahashi JS (2005) Real-time luminescence reporting of circadian gene expression in mammals. Methods Enzymol 393: 288-301.

<Publications using this transgenic line>
Yamazaki S, Numano R, Abe M, Hida A, Takahashi RI, Ueda M, Block GD, Sakaki Y, Menaker M and Tei H (2000) Resetting central and peripheral circadian oscillators in transgenic rats. Science 288: 682-685.

Stokkan K-A, Yamazaki S, Tei H, Sakaki Y and Menaker M (2001) Entrainment of the circadian clock in the liver by feeding. Science 291: 490-493.

Abe M, Herzog ED, Yamazaki S, Straume M, Tei H, Sakaki Y, Menaker M, Block GD (2002) Circadian rhythms in isolated brain regions. J Neurosci 22: 356-356.

Davidson AJ, Stokkan K-A, Yamazaki S, Menaker M (2002) Food-anticipatory activity and liver Per1-luciferase expression in diabetic transgenic rats. Physiol Behav 76: 21-26.

Yamazaki S, Straume M, Tei H, Sakaki Y, Menaker M, Block GD (2002) Effects of aging on central and peripheral mammalian clocks. Proc Natl Acad Sci USA 99: 10801-10806.

Vansteensel MJ, Yamazaki S, Albus H, Deboer T, Block GD, Meijer JH, (2003) Dissociation between circadian Per1, neuronal and behavioral rhythms following a shifted environmental cycle. Curr Biol 13:1538-1542

Yamazaki S, Yoshikawa T, Biscoe EW, Numano R, Gallaspy LM, Soulsby S, Papadimas E, Pezuk P, Doyle SE, Tei H, Sakaki Y, Block GD, Menaker M (2009) Ontogeny of Circadian Organization in the Rat. J Biol Rhythms 24:55-63.

Stokkan K-A, Yamazaki S, Tei H, Sakaki Y and Menaker M (2001) Entrainment of the circadian clock in the liver by feeding. Science 291: 490-493.

Abe M, Herzog ED, Yamazaki S, Straume M, Tei H, Sakaki Y, Menaker M, Block GD (2002) Circadian rhythms in isolated brain regions. J Neurosci 22: 356-356.



Davidson AJ, Stokkan K-A, Yamazaki S, Menaker M (2002) Food-anticipatory activity and liver Per1-luciferase expression in diabetic transgenic rats. Physiol Behav 76: 21-26.

Yamazaki S, Straume M, Tei H, Sakaki Y, Menaker M, Block GD (2002) Effects of aging on central and peripheral mammalian clocks. Proc Natl Acad Sci USA 99: 10801-10806

Vansteensel MJ, Yamazaki S, Albus H, Deboer T, Block GD, Meijer JH, (2003) Dissociation between circadian Per1, neuronal and behavioral rhythms following a shifted environmental cycle. Curr Biol 13:1538-1542

Yamazaki S, Yoshikawa T, Biscoe EW, Numano R, Gallaspy LM, Soulsby S, Papadimas E, Pezuk P, Doyle SE, Tei H, Sakaki Y, Block GD, Menaker M (2009) Ontogeny of Circadian Organization in the Rat. J Biol Rhythms 24:55-63.