Japanese
 NBRP Rat No: 0685 Strain Name: W-Tg(Thy1-COP4/YFP*)4Jfhy Commmon Name: W-TChR2V4, W-Tg(Thy1.2-ChR2Venus)4Jfhy　
 Principal Investigator  Hiromu Yawo
 Organization   Tohoku University Graduate School of Life Sciences
 Address  2-1-1, Katahira, Aoba-ku, Sendai-shi

980-8577  Miyagi

  Japan
 Telephone  022-217-6208  Fax:  022-217-6211  hiromu.yawo.c7@tohoku.ac.jp （担当　五十嵐 敬幸：hirobio@med.tohoku.ac.jp）
 Inbred Generations   10 
   
 Coat Color
 Deposition Status
 
 白
  Embryo      Sperm      Live Animals
 Usage Restrictions  Any form of publications of data resulting from the use of the BIOROGICAL RESOURCE should reference the following DEPOSITOR’s papers.
“Tomita, H., Sugano, E., Fukazawa, Y., Isago, H., Sugiyama, Y., Hiroi, T., Ishizuka, T., Mushiake, H., Kato, M., Hirabayashi, M., Shigemoto, R., Yawo, H. and Tamai, M. Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter. PLoS ONE 4(11), e7679 (2009)”
In publishing, an acknowledgment to the DEPOSITOR is requested.
The BIOROGICAL RESOURCE should be used under the following rules.
1) Any profit organizations cannot use the BIOLOGICAL RESOURCE without contracting license agreements with TOHOKU University.
2) All characteristics of the BIOLOGICAL RESOURCE are not fully known since it was obtained through scientific researches. The recipients should agree that TOHOKU University and the DEPOSITOR will not provide any assurance for the BIOLOGICAL RESOURCE and will not bear any responsibility for the outcome of the use of the BIOLOGICAL RESOUCES without Obligation for Compensation.
3) Either the BIOLOGICAL RESOURCE or the biological materials included in the BIOLOGICAL RESOURCES should not be used in humans or in the edibles creatures even for the diagnosis.
4) The BIOLOGICAL RESOURCE should not be redistributed without the permission by TOHOKU University. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene オワンクラゲGFPの改変体、チャネルロドプシン2(ChR2)
 Origin The transgenic rats were established by injection of transgene consisting of ChR2 and Venus fused transgene driven by the Thy-1.2 promoter into Wistar rat fertile eggs.
 
 Strain Characteristics Thy-1.2 activated cells in these strains can express ChR2-Venus fused protein and seem to have a sensitibity to the light.
 
 Breeding Conditions Good breeding performance.
 
 Genotyping (1) PCR: detection of 720 bp band for Venus. Primers are Venus-F: 5’-atggtgagcaagggcgaggagctgt-3’ (25-mer), Venus-R: 5’-ttacttgtacagctcgtccatgccga-3’ (26-mer).

(2) fluorescence detection using fresh tail sample: Venus fluorescence can be detected in myelinated nerve fiber. 
 References  Tashima R, Koga K, Sekine M, Kanehisa K, Kohro Y, Tominaga K, Matsushita K, Tozaki-Saitoh H, Fukazawa Y, Inoue K, Yawo H, Furue H, Tsuda M.
Optogenetic Activation of Non-Nociceptive Aβ Fibers Induces Neuropathic Pain-like Sensory and Emotional Behaviors after Nerve Injury in Rats.
eNeuro. 2018 Feb 15;5(1). pii: ENEURO.0450-17.2018.

Abe K, Yawo H.
Optogenetic conditioning of paradigm and pattern discrimination in the rat somatosensory system.
PLoS One. 2017 Dec 21;12(12):e0189439.

Lee W, Kim D, Matsuhisa N, Nagase M, Sekino M, Malliaras GG, Yokota T, Someya T.
Transparent, conformable, active multielectrode array using organic electrochemical transistors.
Proc Natl Acad Sci U S A. 2017 Sep 18. pii: 201703886

Jimbo Y, Matsuhisa N, Lee W, Zalar P, Jinno H, Yokota T, Sekino M, Someya T.
Ultraflexible Transparent Oxide/Metal/Oxide Stack Electrode with Low Sheet Resistance for Electrophysiological Measurements.
ACS Appl Mater Interfaces. 2017 Oct 11;9(40):34744-34750.

Lee W, Kim D, Rivnay J, Matsuhisa N, Lonjaret T, Yokota T, Yawo H, Sekino M, Malliaras G. G and Someya T.
Integration of Organic Electrochemical and Field-Effect Transistors for Ultra flexible, High Temporal Resolution Electrophysiology Arrays.
Adv. Mater. 2016, DOI: 10.1002/adma.201602237

Tomita H, Sugano E, Fukazawa Y, Isago H, Sugiyama Y, Hiroi T, Ishizuka T, Mushiake H, Kato M, Hirabayashi M, Shigemoto R, Yawo H, Tamai M (2009) Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter. PLoS One. Nov 5;4(11):e7679.

Ji Z-G, Ito S, Honjoh T, Ohta H, Ishizuka T, Fukazawa Y, Yawo H (2012) Light-evoked somatosensory perception of transgenic rats which express channelrhodopsin-2 in dorsal root ganglion cells. PLoS ONE 7:e32699.