Japanese
 NBRP Rat No: 0687 Strain Name: W-Tg(Thy1-COP4/YFP*)6Jfhy Commmon Name: W-TChR2V6, W-Tg(Thy1.2-ChR2Venus)6Jfhy
 Principal Investigator  Hiromu Yawo
 Organization   Tohoku University Graduate School of Life Sciences
 Address  2-1-1, Katahira, Aoba-ku, Sendai-shi

980-8577  Miyagi

  Japan
 Telephone  022-217-6208  Fax:  022-217-6211  hiromu.yawo.c7@tohoku.ac.jp （担当　五十嵐 敬幸：hirobio@med.tohoku.ac.jp）
 Inbred Generations   3 
   
 Coat Color
 Deposition Status
 
 白
  Embryo      Sperm      Live Animals
 Usage Restrictions  Any form of publications of data resulting from the use of the BIOROGICAL RESOURCE should reference the following DEPOSITOR’s papers.
“Tomita, H., Sugano, E., Fukazawa, Y., Isago, H., Sugiyama, Y., Hiroi, T., Ishizuka, T., Mushiake, H., Kato, M., Hirabayashi, M., Shigemoto, R., Yawo, H. and Tamai, M. Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter. PLoS ONE 4(11), e7679 (2009)”
In publishing, an acknowledgment to the DEPOSITOR is requested.
The BIOROGICAL RESOURCE should be used under the following rules.
1) Any profit organizations cannot use the BIOLOGICAL RESOURCE without contracting license agreements with TOHOKU University.
2) All characteristics of the BIOLOGICAL RESOURCE are not fully known since it was obtained through scientific researches. The recipients should agree that TOHOKU University and the DEPOSITOR will not provide any assurance for the BIOLOGICAL RESOURCE and will not bear any responsibility for the outcome of the use of the BIOLOGICAL RESOUCES without Obligation for Compensation.
3) Either the BIOLOGICAL RESOURCE or the biological materials included in the BIOLOGICAL RESOURCES should not be used in humans or in the edibles creatures even for the diagnosis.
4) The BIOLOGICAL RESOURCE should not be redistributed without the permission by TOHOKU University. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene オワンクラゲGFPの改変体、チャネルロドプシン2(ChR2)
 Origin The transgenic rats were established by injection of transgene consisting of ChR2 and Venus fused transgene driven by the Thy-1.2 promoter into Wistar rat fertile eggs.
 
 Strain Characteristics The transgenic rats were established by injection of transgene consisting of ChR2 and Venus fused transgene driven by the Thy-1.2 promoter into Wistar rat fertile eggs.
 
 Breeding Conditions Good breeding performance.
 
 Genotyping (1) PCR: detection of 720 bp band for Venus. Primers are Venus-F: 5’-atggtgagcaagggcgaggagctgt-3’ (25-mer), Venus-R: 5’-ttacttgtacagctcgtccatgccga-3’ (26-mer).

(2) fluorescence detection using fresh tail sample: Venus fluorescence can be detected in myelinated nerve fiber. 
 References  Tomita H, Sugano E, Fukazawa Y, Isago H, Sugiyama Y, Hiroi T, Ishizuka T, Mushiake H, Kato M, Hirabayashi M, Shigemoto R, Yawo H, Tamai M (2009) Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter. PLoS One. Nov 5;4(11):e7679.