Japanese
 NBRP Rat No: 0679 Strain Name: DA.PVG.1AV1-(D17Rat8-D17Rat37)/Kini Commmon Name: DA.PVG.1AV1-Eae23
 Principal Investigator  Tomas Olsson
 Organization   Karolinska Institutet 
 Address  CMM, Karolinska University Hospital, 171 76,

L8:04, Stockholm

  Sweden
 Telephone  +46 8 51776258  Fax:  +46 8 51775562  tomas.olsson@ki.se
 Inbred Generations   Congenic strain was establiesh using a “speed congenic” strategy in 2008. Since then, the strain was kept as inbred.  
   
 Coat Color
 Deposition Status
 
 Agouti
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development and the characterization of the strain has been published in the following article:
Stridh, P Thessen Hedreul, M Beyeen, AD Adzemovic, MZ Laaksonen, H Gillett, A Ockinger, J Marta, M Lassmann, H Becanovic, K Jagodic, M Olsson, T. Fine-mapping resolves Eae23 into two QTLs and implicates ZEB1 as a candidate gene regulating experimental neuroinflammation in rat. PLos One, 2010 Sep 15;5(9):e12716. PMID:20856809. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others Immune and Inflammatory Diseases, Neurological Diseases (Brain Inflammation and Demyelination)
  Control Strains   Reporter gene Strains  
 Gene > 47 Mb
 Origin Inbred Dark Agouti (DA) rats were originally obtained from the Zentralinstitut für Versuchstierzucht (Hannover, Germany) and Piebald Virol Glaxo(PVG) rats from Harlan UK Limited (Blackthorn, UK). Congenic strain was establiesh using a “speed congenic” strategy (Wakeland et al. 1997) with 8 microsatellite markers from the telomeric marker D17Rat8 to D17Rat37, and 95 microsatellite markers outside the congenic regions. In the N7 generation, all 95 background markers were fixed as DA homozygous. One further backcross was performed and chromosome 17 (Eae23) heterozygous rats subsequently intercrossed to produce the congenic strain DA.PVG.1AV1-(D17Rat8-D17Rat37) in 2008. Since then, the strain was kept as inbred.  
 Strain Characteristics Partial protection against experimental autoimmune encephalomyelitis (EAE) compared to the susceptible parental DA strain (no significant difference in susceptibility (INC and ONS), but DA.PVG-Eae23 rats had lower severity. CNS has increased level of Foxp3 positive cells. 
 Breeding Conditions Good breeding performance.  
 Genotyping Flanking markers inside the congenic fragment: D17Rat8 and D17Rat37. Flanking markers outside the congenic fragment: D17Rat6 and D17Rat172. 
 References  Stridh, P Thessen Hedreul, M Beyeen, AD Adzemovic, MZ Laaksonen, H Gillett, A Ockinger, J Marta, M Lassmann, H Becanovic, K Jagodic, M Olsson, T. Fine-mapping resolves Eae23 into two QTLs and implicates ZEB1 as a candidate gene regulating experimental neuroinflammation in rat. PLoS One. 2010 Sep 15;5(9):e12716. PMID:20856809.