Japanese
 NBRP Rat No: 0682 Strain Name: DA.PVG.1AV1-(D10Rat81-D10Rat238)/Kini Commmon Name: DA.PVG.1AV1-Eae18a
 Principal Investigator  Tomas Olsson
 Organization   Karolinska Institutet 
 Address  CMM, Karolinska University Hospital, 171 76,

L8:04, Stockholm

  Sweden
 Telephone  +46 8 51776258  Fax:  +46 8 51775562  tomas.olsson@ki.se
 Inbred Generations   Congenic strain was establiesh from the 10th generation advanced intercross line after 10 generations of backcrossing in 2010. Since then, the strain was kept as inbred.  
   
 Coat Color
 Deposition Status
 
 Agouti
  Embryo      Sperm      Live Animals
 Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others Immune and Inflammatory Diseases, Neurological Diseases (Brain Inflammation and Demyelination)
  Control Strains   Reporter gene Strains  
 Gene > 13.0 Mb
 Origin Inbred Dark Agouti (DA) rats were originally obtained from the Zentralinstitut für Versuchstierzucht (Hannover, Germany) and Piebald Virol Glaxo(PVG) rats from Harlan UK Limited (Blackthorn, UK). Animals for congenic breeding were selected from the 10th generation of an advanced intercross line (AIL) originating from the EAE-susceptible DA and EAE-resistant PVG.av1 rat strains. Selected males containing a PVG.av1 fragment in the Eae18a region (from D10Rat81 to D10Rat238 marker, at 49.62 Mb and 62.68 Mb, respectively) were backcrossed with DA females for 10 generations, and offspring were genotyped using microsatellite markers in each generation. In the 10th generation, heterozygous males and females were crossed to obtain the experimental population of homozygous congenic rats and littermate controls. 
 Strain Characteristics  
 Breeding Conditions Good breeding performance.  
 Genotyping Flanking markers inside the congenic fragment: D10Rat81-D10Rat238. 
 References  Jagodic M, Becanovic K, Sheng JR, Wu X, Bäckdahl L, Lorentzen JC, Wallström E, Olsson T. An advanced intercross line resolves Eae18 into two narrow quantitative trait loci syntenic to multiple sclerosis candidate loci. J Immunol. 2004 Jul 15;173(2):1366-73. PMID: 15240732. Ockinger J, Serrano-Fernández P, Möller S, Ibrahim SM, Olsson T, Jagodic M. Definition of a 1.06-Mb region linked to neuroinflammation in humans, rats and mice. Genetics. 2006 Jul;173(3):1539-45. Epub 2006 Apr 19. PMID: 16624898.