Japanese
 NBRP Rat No: 0697 Strain Name: DA.ACI-(D10Rat2-D10Rat29)/Kini Commmon Name: DA.ACI-Eae18
 Principal Investigator  Tomas Olsson
 Organization   Karolinska Institutet 
 Address  CMM, Karolinska University Hospital, 171 76,

L8:04, Stockholm

  Sweden
 Telephone  +46 8 51776258  Fax:  +46 8 51775562  tomas.olsson@ki.se
 Inbred Generations   Congenic strain was establiesh using a “speed congenic” strategy as N6F1 in 1999. Since then, the strain was kept as inbred.  
   
 Coat Color
 Deposition Status
 
 Agouti
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development and the characterization of the strain has been published in this article:
Jagodic M, Kornek B, Weissert R, Lassmann H, Olsson T, Dahlman I. Congenic mapping confirms a locus on rat chromosome 10 conferring strong protection against myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis.Immunogenetics. 2001 Jul;53(5):410-5. PMID: 11486278. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene > 50 Mb
 Origin Inbred Dark Agouti (DA) rats originally obtained from Zentralinstitut for Versuchstierzucht (Hannover, Germany) and AxC 9935 Irish (ACI) rats from Harlan Sprague Dawley Inc. (Indianapolis, USA). Congenic strain was establiesh using a “speed congenic” strategy (Wakeland et al. 1997) with 8 microsatellite markers from the telomeric marker D10Rat2 to D10Rat29, and 70 microsatellite markers outside the congenic regions. In the N5 generation, all 70 background markers were fixed as DA homozygous. One further backcross was performed and chromosome 10 heterozygous rats subsequently intercrossed to produce the congenic strain DA.ACI (N6F1) in 1999. Since then, the strain was kept as inbred.  
 Strain Characteristics Partial protection against experimental autoimmune encephalomyelitis (EAE) compared to the susceptible parental DA strain. 
 Breeding Conditions Good breeding performance.  
 Genotyping Flanking markers inside the congenic fragment: D10Rat2 and D10Rat29. 
 References  Jagodic M, Kornek B, Weissert R, Lassmann H, Olsson T, Dahlman I. Congenic mapping confirms a locus on rat chromosome 10 conferring strong protection against myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis.Immunogenetics. 2001 Jul;53(5):410-5. PMID: 11486278.