Japanese
 NBRP Rat No: 0701 Strain Name: DA.PVG.1AV1-(D4Kini3-D4Mgh14)/Kini Commmon Name: DA.PVG.1AV1-Hse1-R1
 Principal Investigator  Tomas Olsson
 Organization   Karolinska Institutet 
 Address  CMM, Karolinska University Hospital, 171 76,

L8:04, Stockholm

  Sweden
 Telephone  +46 8 51776258  Fax:  +46 8 51775562  tomas.olsson@ki.se
 Inbred Generations   Congenic strain was establiesh using a “speed congenic” strategy as N5F1 in 2009. Since then, the strain was kept as inbred.  
   
 Coat Color
 Deposition Status
 
 Agouti
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development and the characterization of the strain has been published in this article:
Abdelmagid N, Bereczky-Veress B, Guerreiro-Cacais AO, Bergman P, Luhr KM, Bergström T, Sköldenberg B, Piehl F, Olsson T, Diez M. The Calcitonin Receptor Gene Is a Candidate for Regulation of Susceptibility to Herpes simplex Type 1 Neuronal Infection Leading to Encephalitis in Rat. PLoS Pathog. 2012 Jun;8(6):e1002753. Epub 2012 Jun 28. PMID: 22761571. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others Viral encephalitis (HSE)
  Control Strains   Reporter gene Strains  
 Gene > 9Mb
 Origin Inbred Dark Agouti (DA) rats were originally obtained from the Zentralinstitut für Versuchstierzucht (Hannover, Germany) and Piebald Virol Glaxo(PVG) rats from Harlan UK Limited (Blackthorn, UK). The congenic line was established from DA and MHC-identical PVG.A rats using a speed-congenic approach with marker assisted selection. DA females were mated with male offspring selected from F2 (DAxPVG.A) with heterozygote alleles within chromosome 4 and Hse1 QTL interval and against lower PVG.A background contamination using 86 microsatellite markers equally spaced throughout the genome (at 20 centimorgan cM intervals). A breeding pair selected from the N5 generation were crossed for two generations to produce homozygous DA.PVGc4-Hse1-R1 congenic rats, carrying PVG alleles between D4Kini3 at 27.81 Mb and D4Mgh14 at 36.5 Mb. 
 Strain Characteristics Protected from Herpes Simplex Encephalitis (HSE) development in 45 days old rats. 
 Breeding Conditions Good breeding performance.  
 Genotyping Flanking markers inside the congenic fragment: D4Kini3 - D4Mgh14. 
 References  Abdelmagid N, Bereczky-Veress B, Guerreiro-Cacais AO, Bergman P, Luhr KM, Bergström T, Sköldenberg B, Piehl F, Olsson T, Diez M. The Calcitonin Receptor Gene Is a Candidate for Regulation of Susceptibility to Herpes simplex Type 1 Neuronal Infection Leading to Encephalitis in Rat. PLoS Pathog. 2012 Jun;8(6):e1002753. Epub 2012 Jun 28. PMID: 22761571.