Japanese
 NBRP Rat No: 0703 Strain Name: DA.BN-(D9Wox24-D9Rat44)/Kini Commmon Name: DA.BN-Eae4-R25
 Principal Investigator  Tomas Olsson
 Organization   Karolinska Institutet 
 Address  CMM, Karolinska University Hospital, 171 76,

L8:04, Stockholm

  Sweden
 Telephone  +46 8 51776258  Fax:  +46 8 51775562  tomas.olsson@ki.se
 Inbred Generations   Congenic strain was establiesh using a “speed congenic” strategy as N6F1 in 1999. Subsequently, interval-specific recombinants were bred from an F2 breeding. R25 recombinant was established in 2003. Since then, the strain was kept as inbred.  
   
 Coat Color
 Deposition Status
 
 Agouti
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development and the characterization of the strain has been published in this article:
Jagodic M, Colacios C, Nohra R, Dejean AS, Beyeen AD, Khademi M, Casemayou A, Lamouroux L, Duthoit C, Papapietro O, Sjöholm L, Bernard I, Lagrange D, Dahlman I, Lundmark F, Oturai AB, Soendergaard HB, Kemppinen A, Saarela J, Tienari PJ, Harbo HF, Spurkland A, Ramagopalan SV, Sadovnick DA, Ebers GC, Seddighzadeh M, Klareskog L, Alfredsson L, Padyukov L, Hillert J, Clanet M, Edan G, Fontaine B, Fournié GJ, Kockum I, Saoudi A, Olsson T. A role for VAV1 in experimental autoimmune encephalomyelitis and multiple sclerosis. Sci Transl Med. 2009 Dec 9;1(10):10ra21. PMID:20368159.  
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene few Mb (positionally cloned to Vav1 gene using interval-specific congenic lines)
 Origin Inbred Dark Agouti (DA) and Brown Norway (BN) rats were originally obtained from the Zentralinstitut für Versuchstierzucht (Hannover, Germany). Congenic strain was establiesh using a “speed congenic” strategy (Wakeland et al. 1997). Briefly, in each backcross generation, rats for further breeding were selected on the basis of genotyping of 8 microsatellite markers in the congenic region, from marker D9Wox24 to D9Rat20. The genetic background of rats was also screened with 100 markers. After the complete removal of the donor genome outside the congenic fragment, two heterozygous rats were intercrossed to produce the homozygous strains DA.BN-Eae4(N7F1). Subsequently, interval-specific recombinants were bred from an F2 breeding. R25 recombinant was established in 2003. 
 Strain Characteristics Protection against experimental autoimmune encephalomyelitis (EAE) compared to the susceptible parental DA strain, regulation of proinflammatory T cell activation and regulatory T cells. 
 Breeding Conditions Good breeding performance.  
 Genotyping Flanking markers inside the congenic fragment: D9Wox24-D9Rat44. 
 References  Jagodic M, Colacios C, Nohra R, Dejean AS, Beyeen AD, Khademi M, Casemayou A, Lamouroux L, Duthoit C, Papapietro O, Sjöholm L, Bernard I, Lagrange D, Dahlman I, Lundmark F, Oturai AB, Soendergaard HB, Kemppinen A, Saarela J, Tienari PJ, Harbo HF, Spurkland A, Ramagopalan SV, Sadovnick DA, Ebers GC, Seddighzadeh M, Klareskog L, Alfredsson L, Padyukov L, Hillert J, Clanet M, Edan G, Fontaine B, Fournié GJ, Kockum I, Saoudi A, Olsson T. A role for VAV1 in experimental autoimmune encephalomyelitis and multiple sclerosis. Sci Transl Med. 2009 Dec 9;1(10):10ra21. PMID:20368159.

Colacios C, Casemayou A, Dejean AS, Gaits-Iacovoni F, Pedros C, Bernard I, Lagrange D, Deckert M, Lamouroux L, Jagodic M, Olsson T, Liblau RS, Fournié GJ, Saoudi A. The p.Arg63Trp polymorphism controls Vav1 functions and Foxp3 regulatory T cell development. J Exp Med. 2011 Oct 24;208(11):2183-91. Epub 2011 Sep 26. PMID: 21948080.